Legionella pneumophila is a Gram-negative bacterial pathogen that can cause a severe form of pneumonia called Legionnaires' disease.A major virulence determinant of *** is the type IVB secretion system (T4SSB),whi...
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Legionella pneumophila is a Gram-negative bacterial pathogen that can cause a severe form of pneumonia called Legionnaires' disease.A major virulence determinant of *** is the type IVB secretion system (T4SSB),which is used to inject more than 300 effector proteins into the host cell,modulating various cellular *** effector,WipA,was the first tyrosine protein phosphatase found in *** with no clear host substrates;moreover,the role of tyrosine phosphorylation during *** infection and the signal pathways responsible for it are relatively ***,liquid chromatography-tandem mass spectrometry for label-free quantitative phosphoproteomics was applied to systematically investigate the global tyrosine phosphorylation events in Legionella-infected pulmonary alveolar *** analysis of the phosphoproteomics data revealed an increase in the tyrosine phosphorylation levels of 44 sites,and a decrease of 36 sites in wild type Lp02 infected macrophages compared to LpO2-△wipA mutant infected *** uncover an early function of WipA,showing that it associates with host actin *** majority of phosphosites were actin assembly signaling pathway associated proteins,including tyrosine kinase signaling regulator Nck1,tyrosine kinase (Lyn and Yes),nuclear promote factor NWASP,actin related protein Arp3,ADP ribosylation factor (Arf) 6 and Capping ***,western blotting further confirmed the decrease in several tyrosine phosphosites phosphorylation levels during ***,we found that WipA inhibited host actin *** findings suggested that the WipA-mediated tyrosine phosphorylation pathways may play important roles in the regulation of actin assembly in host and may help us elucidate the pathogenic mechanisms of *** infection.
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