目的:探讨葛根素(puerarin,Pue)治疗绝经后骨质疏松症(postmenopausal osteoporosis,PMOP)大鼠的效果及作用机制。方法:将雌性SD大鼠分为假手术组(SHAM组)、模型组(PMOP组)、葛根素低剂量组(Pue-L组)和葛根素高剂量组(Pue-H组)。SHAM组取卵巢周围部分脂肪,其余各组切除双侧卵巢,术后4周检测骨矿物质密度(bone mineral density,BMD)和苏木精-伊红(HE)染色法观察股骨组织,判断造模是否成功。造模成功后Pue-L组和Pue-H组大鼠皮下注射不同浓度葛根素注射液,SHAM组和PMOP组注射等量生理盐水。给药8周后检测大鼠BMD;用显微CT(Micro-CT)扫描观察骨结构并计算相关参数;HE染色观察大鼠股骨病理改变;Western Blot(WB)法检测JAK2/STAT3信号通路蛋白表达水平。结果:(1)Pue可显著提高PMOP大鼠BMD。(2)Micro-CT分析显示,Pue可改善PMOP大鼠骨微结构。(3)HE染色显示Pue治疗后PMOP大鼠骨量丢失减少、骨小梁数量与密度增加,病理改变有所恢复。(4)WB检测结果:与SHAM组相比,PMOP组p-JAK2、p-STAT3蛋白表达水平升高(P<0.001);与PMOP组比较,Pue-L组和Pue-H组p-JAK2、p-STAT3蛋白表达水平降低(P<0.001)。结论:葛根素可有效治疗PMOP大鼠,并可能通过抑制JAK2/STAT3通路的激活发挥抗骨质疏松作用。
目的:探究能谱CT对直肠癌淋巴结转移测定的价值。方法:选取包头医学院第二附属医院行腹腔镜下直肠癌根治术的50例患者,共纳入225枚淋巴结,其中转移性淋巴结51枚,非转移性淋巴结174枚。对比分析转移性淋巴结与非转移性淋巴结的长径、短径、长短径之比,同时对各淋巴结的能谱参数进行对比分析。结果:转移性淋巴结组与非转移性淋巴结组的长径、长短径之比具有统计学意义(P Objective: To explore the value of spectral CT in the determination of lymph node metastasis in rectal cancer. Methods: A total of 225 lymph nodes were included in 50 patients who underwent laparoscopic radical rectal cancer resection in the Second Affiliated Hospital of Baotou Medical College, including 51 metastatic lymph nodes and 174 non-metastatic lymph nodes. The ratios of long diameter, short diameter and long short diameter of metastatic lymph nodes and non-metastatic lymph nodes were compared and analyzed, and the energy spectrum parameters of each lymph node were compared and analyzed. Results: The ratio of length to diameter between metastatic lymph node group and non-metastatic lymph node group was statistically significant (P < 0.05). In arteriovenous phase, IC, NIC and substance were statistically significant compared with Water-Iodine, Iodine-Fe, Fe-Iodine, Iodine-Cu, Cu-Iodine and Blood-Iodine (P < 0.05). Conclusion: Spectral CT has a high differential value in determining whether lymph nodes metastasize in rectal cancer.
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