The full\|length cDNA of mosquito esterase B1 had been isolated,and subcloned into *** recombinant vector pBV220B1 was constructed and transformed into *** DH5α.A 60 kD protein was induced by 42℃ and its expression ...
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The full\|length cDNA of mosquito esterase B1 had been isolated,and subcloned into *** recombinant vector pBV220B1 was constructed and transformed into *** DH5α.A 60 kD protein was induced by 42℃ and its expression was temperature\|*** 6h induction,the target protein occupied 50% of the total *** expressed product existed in both inclusion body and soluble proteins in the *** amount of the soluble detoxifying enzyme increased along with the induction *** data of detoxifying experiments indicated that the detoxifying enzyme in expression strain of *** can detoxified toxicity of organophosphate insecticides,it showed a clear detoxifying affect on hens poisoned by organophosphate insecticides.
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