According to the conservative sequence in the epitaxial variable region of Thailand strain of WSSV published in GenBank,a pair of primers were designed to amplify the variable region genes of 5 local WSSV strains(ZHSH...
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According to the conservative sequence in the epitaxial variable region of Thailand strain of WSSV published in GenBank,a pair of primers were designed to amplify the variable region genes of 5 local WSSV strains(ZHSH,ZHJ,HN,QD1,QD2) by PCR and then *** accordance with the CN,the results indicated that the number of nucleotide of 5 strains were deleted more than 591bp of the TW and TH *** ZHSH and HN strains that deleted 591bp at the 3′ end,and 454 bp at the 5′ end of varible gene was highly homologous with CN strain about 99.3%.229bp of ZHJ strain at the 5′ end was homologous with CN about 99.3%,and deleted of 816bp at the 3′ end.97bp at the 5′ end and 171bp at the 3′end of QD1 and QD2 strains were homologous with CN strain about 99.3%,and about 777bp were absent in the *** above data showed that the variable region genes of WSSV had mutated more in *** variable region gene of QD2 strain was coincidence with that of QD1 after propagating 10 generations in *** results indicated that the crayfish inoculation did not result in mutation of variable region genes of WSSV.
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