The GP5 gene which was spanned or truncated with hydrophobic signal peptide(tGP5) was amplified with RT-PCR and cloned into the downstream of promoter(CMV) of the pcDNA3.1,an eukaryotic expressing *** constructed plas...
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The GP5 gene which was spanned or truncated with hydrophobic signal peptide(tGP5) was amplified with RT-PCR and cloned into the downstream of promoter(CMV) of the pcDNA3.1,an eukaryotic expressing *** constructed plasmids were transfected into HEK-293A cells with anion lipid transfection ***,the expression of the mRNA of interest gene was analyzed and confirmed by RT-PCR,and the expression of target protein was confirmed by indirect immunofluorescent assay(IFA).Each mouse was immunized with 100 μg of naked plasmid by intradermal *** groups were immunized four times with interval of 15 *** neutralizing antibody against PRRSV were induced,but the antibody of group immunized with tGP5 gene produced slowly and the titer was lower than that in the group immunized with GP5 *** showed that the signal peptide of the GP5 protein of PRRSV is associated with the neutralizing antibody level of DNA immunization of pertinent GP5 gene.
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