Electron microscopic examinations of ultrathin sections reveal the presence of spherical virus like particles in the digestive gland, kidney, and the intestine of moribund scallop Chlamys farreri collected during a se...
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Electron microscopic examinations of ultrathin sections reveal the presence of spherical virus like particles in the digestive gland, kidney, and the intestine of moribund scallop Chlamys farreri collected during a severe mortality in Jiaozhou Bay (Qingdao, China) in 2000. The virions are approximately 130 to 170 nm in diameter and have a bilaminal envelope, while the nucleocapsids are 90-140 nm. The virus occurs and distributes in the cytoplasmic vesicles of infected cells, and it is arranged in a scattered form without occluding protein. The viral particles are consistent in size and morphology in preparations derived from the isolation and also from ultrathin sections. The spikes of envelope are clearly observed with negative staining. By the same methods, this virus was not found in the healthy scallop, indicating that this virus might be the causative aetiology for the abnormal mortality.
The current status and importance of preserving fish germplasm resources were described. The basic principle of cryobiology and its application potentials in preservation of aquatic germplasm resources were examined. ...
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The current status and importance of preserving fish germplasm resources were described. The basic principle of cryobiology and its application potentials in preservation of aquatic germplasm resources were examined. The mechanism of cryodamage was discussed. The recent advances and major problems in finfish gamete and embryo cryopreservation were reviewed. The prospects for cryopreservation research in fish gametes and embryos were looked into. The important research directions in cryopreservation of aquatic organisms were proposed.
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