Membrane voltage is an important biophysical signal. Optical mapping of membrane voltage enables investigation of electrical signaling at high spatial resolutions and with high throughput. Here, we present a novel flu...
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Membrane voltage is an important biophysical signal. Optical mapping of membrane voltage enables investigation of electrical signaling at high spatial resolutions and with high throughput. Here, we present a novel fluorescent voltage indicator scaffold(FlareFRET) that builds upon site-specific modification of microbial rhodopsin with organic fluorophores. FlareFRET achieved 36% ΔF/F per100 mV voltage change with sub-millisecond response kinetics, thus representing one of the most sensitive and fastest orange-colored voltage indicators. This technique has enabled observation of long-range electrical coupling among mammalian cells that was mediated via gap-junctions. Combining the superior brightness and photostability of small molecules with genetic targeting of microbial rhodopsin proteins, the FlareFRET design strategy can be extended for developing novel fluorescent indicators.
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