The electronic structure of protein chains L and M in photosynthetic reaction center (PRC) of Rhodobacter sphaeroides (Van Niel) Imhoff, Truper et Pfennig) was studied by using the Overlapping Dimer Approximation meth...
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The electronic structure of protein chains L and M in photosynthetic reaction center (PRC) of Rhodobacter sphaeroides (Van Niel) Imhoff, Truper et Pfennig) was studied by using the Overlapping Dimer Approximation method and the Extended Negative Factor Counter method at ab initio level. The result indicated that: (1) Amino acid residues, the molecular orbitals of which composed the main components of frontier orbitals of protein chain L (M), are located at the random coil areas of chain L (alpha helix areas of chain M). Since the random coil is flexible and more easy to change its conformation in the electron transfer process and to reduce the energy of the system, and the structure of the alpha helix is reletively stable, this difference might be one of the causes for the electron transfer in photosynthetic reaction center (PRC) only takes place along the L branch. (2) The His residues which axially coordinated to the 'special pair' P and accessory chlorophyll molecules (ABChls) are essentially important for the E-LUMO levels of P and ABChl. But, the corresponding molecular orbitals of these His residues do not appear in the composition of frontier orbitals of protein chains. It means that the interaction between pigment molecules and protein chains do not influence the contribution to the frontier orbitals of protein chains explicitly, but influences the corresponding E-LUMO levels significantly.
Synthesis of a novel α phosphorus containing spin trap, DEPPEPO[5 (diethoxyphosphoryl) 5 phenylethyl 1 pyrroline] N oxide and evaluation of its ability to spin trap the hydroxyl and the superoxide anion radicals were...
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Synthesis of a novel α phosphorus containing spin trap, DEPPEPO[5 (diethoxyphosphoryl) 5 phenylethyl 1 pyrroline] N oxide and evaluation of its ability to spin trap the hydroxyl and the superoxide anion radicals were described in the present article. The ability for trapping an active superoxide anion radicals generated in the HX/XO system and the stability of their spin adduct of DEPPEPO were analyzed. In addition, the effects of 5 phenylethyl and 5 phenyl substituted analogues of DMPO on the stereochemistry of the corresponding spin adducts were systematically illustrated.
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