目的确认TrkA与snapin蛋白间的直接相互作用。方法采用DNA重组技术,构建增强型荧光蛋白表达载体pECFP-TrkAICD(TrkA膜内区)和pEYFP-snapin,共转染HEK 293T细胞后以激光扫描共聚焦显微镜观察并进行荧光共振能量转移(fluorescence resonance energy transfer,FRET)分析。结果成功构建了snapin和TrkA的重组质粒,共转染细胞后激光扫描共聚焦显微镜分析表明两种蛋白分布在细胞质同一层面,荧光共振能量转移(FRET)分析表明能量转移效率>5%,与对照相比有显著区别(P<0.05)。结论激光扫描共聚焦及FRET实验结果都证明了TrkA膜内区与snapin两个蛋白之间存在着直接的相互作用。
The nervous system is a complex network with many types of neurons and numerous synaptic *** present knowledge on how neurons recognize specific targets and form such an intricate network is still *** Down syndrome ce...
详细信息
The nervous system is a complex network with many types of neurons and numerous synaptic *** present knowledge on how neurons recognize specific targets and form such an intricate network is still *** Down syndrome cell adhesion molecule （DSCAM） belongs to the immunoglobulin superfamily and contributes to defects in the central nervous system in Down syndrome *** plays important roles in neural development,including dendritic patterning and self-avoidance,axon guidance and branching,axon target recognition and synaptic ***,the functional mechanisms and the underlying signaling pathways are still largely *** the functions of DSCAM in neural development were *** research for better understanding DSCAM function and the relevance of DSCAM to human diseases was also discussed.
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