Retinal ischemia-reperfusion(I/R)injury is associated with many ocular *** cell death,macrophage infiltration,and oxidative injury are complications in retinal I/R ***(SF),an isothiocyanate found in cruciferous vegeta...
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Retinal ischemia-reperfusion(I/R)injury is associated with many ocular *** cell death,macrophage infiltration,and oxidative injury are complications in retinal I/R ***(SF),an isothiocyanate found in cruciferous vegetables such as broccoli,has cytoprotective effects against oxidative stress and its effect is mediated by nuclear factor E2-related factor 2(Nrf2),a transcription factor,and heme oxygenase-1(HO-1),which is one of Nrf2 downstream target *** study was undertaken to investigate the neuroprotective mechanisms of SF in the retina I/R rat ***(12.5mg/kg)or vehicle(corn oil)was intraperitoneally administered once a day for 1 ***,retinal I/R was induced by increasing the intraocular pressure(IOP)to 130 mm Hg for 60 *** specific experiments,protoporphyrin IX zinc(Ⅱ)(Zn PP,30 mg/kg),an HO-1 inhibitor,was intraperitoneally administered at 24 h prior to *** protective effects of SF were evaluated by determining the morphology of the retina,counting the infiltrating inflammatory cells and the surviving retinal ganglion cells(RGCs)and amacrine cells,and measuring apoptosis in the retinal *** expression of Nrf2and HO-1 was examined by immunofluorescence analysis and Western blotting.I/R induced a marked increase of ROS generation,caused pronounced inflammation,increased the apoptosis of RGCs and amacrine cells and caused the thinning of the inner retinal layer(IRL),and these effects were diminished or abolished by SF ***,SF treatment significantly increased the nuclear accumulation of Nrf2 and the level of HO-1 expression in the retinas after I/R injury;however,.Zn PP diminished the protective effects of SF on I/R *** results suggested that SF had protective effects on I/R retinas,which were mediated,at least in part,by the activation of the Nrf2/HO-1 antioxidant pathway.
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