检索结果-内蒙古大学图书馆

药物分析杂志 2006年第9期26卷 1350-1357页

作者：刘一凌笑梅崔景荣北京大学药学院药物分析教研室北京100083 北京大学医学部天然药物及仿生药物国家重点实验室北京100083

毛细管电泳技术是八十年代初发展起来的一种新型分离分析技术。由于其分析时间短,样品消耗少,分离度高,应用灵活,能够在生理条件或者接近生理条件的缓冲液中运行等优点,广泛应用于研究生物分子间的相互作用。本文就毛细管电泳研究生物... 详细信息

毛细管电泳技术是八十年代初发展起来的一种新型分离分析技术。由于其分析时间短,样品消耗少,分离度高,应用灵活,能够在生理条件或者接近生理条件的缓冲液中运行等优点,广泛应用于研究生物分子间的相互作用。本文就毛细管电泳研究生物分子间相互作用的基本原理,及其在生物分子间相互作用和药物筛选中的应用进行了综述。对不同的分子间相互作用体系,如蛋白质-蛋白质/多肽,蛋白质-DNA/RNA,蛋白质-糖,抗原-抗体,蛋白质-药物,脂质体-蛋白质/药物等进行了论述。同时也探讨了毛细管电泳从组合化学库中筛选先导药物及其在药物筛选中的应用。

关键词：毛细管电泳生物分子相互作用药物筛选

来源：评论

学校读者我要写书评

暂无评论

Genome-wide identification of long noncoding RNAs in myocyte differentiation

引用

Journal of Chinese Pharmaceutical Sciences 2017年第6期26卷 423-431页

作者：杨琴李栋冯超易凡李华杜权北京大学医学部药学院天然药物及仿生药物国家重点实验室北京100191 北京大学第三医院妇产科北京100191

Taking advantage of high-throughput sequencing technology, a large number of long noncoding RNAs （lncRNAs） have been recently characterized. Emerging evidence suggests that they play critical roles in diverse biological processes, including the differentiation of skeletal muscle cells. As previous studies have focused mainly on the polyadenylated lncRNAs, the involvement of polyA-rninus IncRNAs in myocyte differentiation remains largely unexplored. To this aspect, their expression and regulation were examined in the present study. During the course of myocyte differentiation of C2C12 cells, polyA-minus RNAs were isolated and analyzed by RNA-seq. In addition to identifying 904 novel polyA-minus lncRNAs, their temporal expression was further characterized in this process. For many lncRNAs, differentiation-specific profiles were revealed. Based on their unique expression profiles, these lncRNAs were grouped into nine regulation categories. Taken together, our study greatly contributed to the identification of a dynamic regulation ofpolyA-minus lncRNAs, and clarified their potential roles in myocyte differentiation.

关键词： LncRNA RNA-Seq Myocyte differentiation

来源：评论

学校读者我要写书评

暂无评论

Determination of metformin in diabetic rat plasma by an improved ion-pair high-performance liquid chromatography: application to a pharmacokinetic study

引用

Journal of Chinese Pharmaceutical Sciences 2012年第3期21卷 211-218页

作者：陈烨李汉青许娇娇酒向飞邓晨辉李新刚李良徐小晴周田彦卢炜北京大学医学部药学院药剂学系北京100191 北京大学医学部、天然药物及仿生药物国家重点实验室北京100191

An efficient and sensitive ion-pair HPLC-UV method using atenolol as internal standard （IS） was developed and validated for the determination of metformin in the plasma of diabetic rats. Plasma samples were deproteinated with 10% （v/v） perchloric acid. Separation was achieved on a UltimateTM AQ-C18 column （250 mm×4.6 mm, 5 μm） with a mobile phase （pH 5.05） composed of acetonitrile-water （31：69, v/v, containing 0.002 M sodium dodecyl sulfate, 0.0125 M potassium dihydrogen phosphate, 0.015 M triethylamine） at a flow rate of 1.0 mL/min. The calibration curve was linear （r〉0.994） between 7.5 and 4000 ng/mL. The lower limit of quantification （LLOQ） was 7.5 ng/mL. The precision was validated and the relative standard deviation was in the range of 1.87% to 15.70%; the accuracy was between 93.98%-106.89%. The mean recoveries were 95.40% and 95.31% for metformin and IS, respectively. The relative error （RE） of stability at different storage conditions was within ±9.00%. This method was used to determine the concentration-time profile of metformin in diabetic rat plasma following an oral administration of metformin at the dose of 10 mg/kg. Our results indicated that ion-pair HPLC-UV method using UltimateTM AQ-C18 column was effective for the pharmacokinetic studies of high polarity compounds like metformin.

关键词： Metformin Ion-pair HPLC Diabetic rat plasma Pharmacokinetics Ultimate^TM AQ-C 18

来源：评论

学校读者我要写书评

暂无评论

Effects of dichloroacetate on the activation of the mitochondrial pathway in C6 cells in vitro

引用

Journal of Chinese Pharmaceutical Sciences 2011年第5期20卷 460-465页

作者：赵欣王欣余克富段瑀李捷思赵炳祥张烜张强北京大学医学部药学院药剂学系北京100191 北京大学医学部天然药物及仿生药物国家重点实验室北京100191

Mitochondria are increasingly recognized as important targets for tumor treatment because of their central roles in apoptotic pathways and cellular metabolism. Dichloroacetate （DCA）, a low molecular weight mitochondria-targeting agent, exhibits potential therapeutic effects for tumors. Based on the effects of DCA on tumor cellular metabolism, we carried out this study to investigate the anti-tumor activity of DCA in C6 glioma cells in vitro. The results showed that DCA was able to increase the activity of pyruvate dehydrogenase （PDH）, induce the production of reactive oxygen species （ROS） and reduce the mitochondrial membrane potential （MMP） in C6 ceils in vitro （P〈0.05 or 0.01）, indicating that the anti-tumor effects of DCA in C6 cells could be through the activation of the mitochondrial pathway. In conclusion, mitochondria could be a viable therapeutic target for the treatment of glioma.

关键词： Mitochondria metabolism Dichloroacetate C6 glioma Pyruvate dehydrogenase Reactive oxygen species Mitochondrial membrane potential

来源：评论

学校读者我要写书评

暂无评论

A sensitive LC-MS/MS method to determine the concentrations of erlotinib and its active metabolite OSI-420 in BALB/c nude mice plasma simultaneously and its application to a pharmacokinetic study

引用

Journal of Chinese Pharmaceutical Sciences 2012年第4期21卷 296-303页

作者：李梦瑶吴琼李汉青宁妙然陈烨李良周田彦卢炜北京大学医学部天然药物及仿生药物国家重点实验室北京100191 北京大学医学部药学院药剂学系北京100191

A simple, rapid and sensitive LC-MS/MS method was developed to quantify erlotinib and its active metabolite, OSI-420, simultaneously in BALB/c nude mice plasma. Erlotinib, OSI-420 and propranolol （internal standard） were extracted from nude mice plasma samples by liquid-liquid extraction. Separation was achieved on a reversed phase ClS column with a mobile phase of acetonitrile-water （35：65, v/v） containing 5 mM ammonium formate （pH = 3.0）. All compounds were monitored by mass spectrometry with electrospray positive ionization. The lower limit of quantification was 0.5 ng/mL for both erlotinib and OSI-420; accuracy was estimated by relative error, which was in the range from 0.07% to 8.00% for erlotinib and -2.83% to 6.67% for OSI-420; precision was validated by relative standard deviation, which was from 2.28% to 15.12% for erlotinib and from 1.96% to 11.50% for OSI-420. This method was applied to a pharmacokinetic study of BALB/c nude mice following oral administration of erlotinib at 12.5 mg/kg. A 2-compartment model was used to fit the pharmacokinetics of erlotinib and 1-compartment model for the pharmacokinetics of OSI-420. The ratio of the active metabolite to parent drug in mice was greater than previously reported in humans and probably reflects interspecies difference in the rate of conversion of erlotinib to OSI-420.

关键词： Erlotinib OSI-420 LC-MS/MS Pharmacokinetics BALB/c nude mice

来源：评论

学校读者我要写书评

暂无评论

Dnmt1 regulates adipogenesis by Cdkn1a methylation

引用

Journal of Chinese Pharmaceutical Sciences 2017年第7期26卷 488-495页

作者：杨益易凡张沛李华杜权北京大学医学部药学院天然药物及仿生药物国家重点实验室北京100191 北京大学第三医院妇产科北京100191

Obesity has recently become a major healthy concern in developed countries. This leads to intensive interest in the mechanism study of adipogenesis, in which epigenetic mechanisms are speculated to play an essential role. To explore the function of Dnmt1, its expression was first profiled during the course of adipocyte differentiation of 3T3-L1 cells. The results revealed a dynamic regulation of its expression at the initiation stage. Knockdown of Dnmt1 compromised the differentiation process and decreased lipid production within the cells. To the aspect of epigenetic regulation, promoter methylation of Cdkn1a was significantly increased at the initiation stage of the differentiation, accompanied by decreased Cdkn1a expression. Furthermore, knockdown of Dnmt1 led to an increased Cdkn1a expression, indicating that Dnmt1 inhibits Cdkn1a expression by promoter methylation. Furthermore, we found that knockdown of Cdkn1a up-regulated the expression of PPARγ and resulted in enhanced adipocyte differentiation. In summary, our results demonstrated that Dnmt1 regulated the process of adipogenesis by methylation of Cdkn1a promoter, suggesting that Cdkn1a played a fundamental role in the prevention of adipocyte hyperplasia.

关键词： Adipogenesis Cdkn1a Dnmt1 DNA methylation

来源：评论

学校读者我要写书评

暂无评论

大鼠血浆中新型α7-烟碱型乙酰胆碱受体正向变构调节剂LD486的液相色谱-质谱串联测定方法学的建立及其药代动力学特征（英文）

引用

Journal of Chinese Pharmaceutical Sciences 2016年第7期25卷 517-525页

作者：黄晓敏焦文宣孙崎王克威北京大学医学部药学院分子与细胞药理学系北京大学医学部天然药物及仿生药物国家重点实验室北京大学医学部药学院化学生物学系

神经型α7-烟碱型乙酰胆碱受体功能的不足与认知缺陷以及多种神经精神障碍相关。在动物模型中,激活α7-烟碱型乙酰胆碱受体可以提高学习记忆能力以及感觉门控。为了增强新型的功能,我们通过筛选发现了激动α7-烟碱型乙酰胆碱受体的正向... 详细信息

神经型α7-烟碱型乙酰胆碱受体功能的不足与认知缺陷以及多种神经精神障碍相关。在动物模型中,激活α7-烟碱型乙酰胆碱受体可以提高学习记忆能力以及感觉门控。为了增强新型的功能,我们通过筛选发现了激动α7-烟碱型乙酰胆碱受体的正向调节剂LD486。在本研究中,我们建立了一个简单可靠且高效的液相色谱质谱联用的方法（LC-MS/MS）并分析了这种新型的正向变构调节剂LD486在大鼠血浆中的浓度以及药代动力学参数。含有LD486的血浆样品用反向C18色谱柱分离,流动相为乙腈和0.1%甲酸水溶液（80:20,v/v）,流速为0.5 mL/min,内标为A1223。液相流出液使用电喷雾离子源（ESI,正离子模式）,质谱多反应监测模式检测,检测离子:LD486为m/z 419.0[M+H];→m/z 169.0,A1223为m/z 400.0[M+H]+→m/z 129.9。LD486在4–4000 ng/m L范围内,线性关系良好,最低定量限为4 ng/m L。在最低定量限时,依然有满意的日内日间精密度,提取回收率,以及基质效应。稳定性测试提示血浆中LD486在反复冻融,22oC室温下放置2小时和12小时,以及–20oC保存2周均保持稳定。我们将此方法成功地应用到测定大鼠血浆中LD486静脉注射（1 mg/kg）以及口服（3 mg/kg和30 mg/kg）后给药途径的药代动力学特征。

关键词：乙酰胆碱受体正向变构调节剂 LD486 液相色谱质谱联用药代动力学大鼠血浆 α7受体通道

来源：评论

学校读者我要写书评

暂无评论

氧化还原响应型树枝状大分子的制备及释药性能研究

引用

功能材料 2016年第1期47卷 1072-1076页

作者：余丽丽尤静姚琳梁飞西安医学院药学院西安710021 北京大学医学部药学院天然药物及仿生药物国家重点实验室北京100191

以乙二胺(EDA)、丙烯酸甲酯(MA)、聚乙二醇单甲醚-1900(mPEG-1900)、胱胺二盐酸盐为原料合成两亲树枝状大分子体系(mPEG-G4.0,mPEG-G(S—S)3.0,mPEG-G(S—S)4.0),通过傅里叶红外(FT-IR)确定聚合物的结构,以阿霉素(DOX)为模型分子研究该... 详细信息

以乙二胺(EDA)、丙烯酸甲酯(MA)、聚乙二醇单甲醚-1900(mPEG-1900)、胱胺二盐酸盐为原料合成两亲树枝状大分子体系(mPEG-G4.0,mPEG-G(S—S)3.0,mPEG-G(S—S)4.0),通过傅里叶红外(FT-IR)确定聚合物的结构,以阿霉素(DOX)为模型分子研究该药物载体的包载和药物释放情况,获得树状大分子的特点、性质及对氧化还原的敏感性。结果表明mPEG-G4.0胶束、mPEG-G(S—S)3.0胶束、mPEG-G(S—S)4.0胶束的粒径均在670nm以下,且均具有良好的药物包载性能。载有DOX的mPEG-G(S—S)3.0,mPEG-G(S—S)4.0胶束在高的浓度GSH条件下药物释放速度较快,具有显著的氧化还原敏感性。

关键词：树枝状大分子氧化还原敏感体外释药阿霉素

来源：评论

学校读者我要写书评

暂无评论

甘遂中麻风树烷型大环二萜类化学成分研究

引用

中国中药杂志 2008年第15期33卷 1836-1839页

作者：陈云利袁丹徐鑫傅宏征北京大学医学部天然药物及仿生药物国家重点实验室北京100083 沈阳药科大学中药学院辽宁沈阳110016

目的:研究甘遂Euphorbia kansui中麻风树烷型大环二萜类的化学成分。方法:采用硅胶、凝胶等色谱技术进行分离,通过NMR等波谱学方法鉴定化合物结构。结果:分离并鉴定出3个麻风树烷型大环二萜,分别为甘遂宁D1(kansuinin D1,1),甘遂宁D(kan... 详细信息

目的:研究甘遂Euphorbia kansui中麻风树烷型大环二萜类的化学成分。方法:采用硅胶、凝胶等色谱技术进行分离,通过NMR等波谱学方法鉴定化合物结构。结果:分离并鉴定出3个麻风树烷型大环二萜,分别为甘遂宁D1(kansuinin D1,1),甘遂宁D(kansuinin D,2)和甘遂宁A(kansuinin A,3)。结论:其中化合物1为新化合物。

关键词：甘遂麻风树烷型大环二萜

来源：评论

学校读者我要写书评

暂无评论

Theβ-directing effect of ionic liquid in mannopyranosylation:a potential access to stereoselective construction of the 1,2-cis-β-D-mannopyranosyl linkage

引用

Journal of Chinese Pharmaceutical Sciences 2011年第6期20卷 549-556页

作者：孙晟马庆孟祥豹李庆李树春黄河清李中军北京大学医学部天然药物及仿生药物国家重点实验室药学院化学生物学系北京100191

We report a novel P-directing effect of imidazolium based ionic liquid,which was observed when we conducted the glycosylation of a 6-O-acetyl-2,3,4-tri-O-benzyl-mannose trichloroacetimidate donor with a modified ionic liquid support acceptor. The mechanism was investigated and a hypothesis was *** optimization of the reaction conditions,an innovative method for the selective construction of 1,2-cis-β-D-mannopyranosyl linkage was developed.

关键词： Ionic liquid supported strategy Glycosylation Stereoselectivity

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：