[Objective] This research aimed to solve the problems of growth and differentiation inhibition of transgenic potato plants caused by antibiotics used for bacteriostasis. [Method] Microtubers were induced using transge...
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[Objective] This research aimed to solve the problems of growth and differentiation inhibition of transgenic potato plants caused by antibiotics used for bacteriostasis. [Method] Microtubers were induced using transgenic potato plants, which had generated shoots and formed transgenic bacteria-free plants. [Result] Among the three transgenic potato varieties, the optimal induction medium for SⅠ and SⅡ were MS＋ 0.5 mg/L of 6-BA ＋ 0.1 mg/L of GA3＋ 150 mg/L of cef, and the optimal induction medium for NT were MS＋ 0.5 mg/L of ZT ＋ 0.1 mg/L of GA3 ＋ 150 mg/L of cef; the optimal differentiation medium for tubers were MS＋ 0.5 mg/L of ZT ＋ 0.1 mg/L of NAA, and the tubers with diameters ranging from 0.5 to 0.7 cm had generated the most shoots. The transgenic bacteria-free plants were cultivated in propagation medium without antibiotics for 30 d with a contamination rate of 0, and the stems of bacteria-free plants were stout with no branching. [Conclusion] This method is simple and could be easily applied for the removal of bacteria, which had cleared away obstacles for the selection and growth of transgenic individuals.
PtDr101基因从三倍体毛白杨(Popu1us tomentosa× P. bolleana)×***]中克隆获得,能够编码NBS-LRR型蛋白,受到水杨酸和甲基茉莉酸诱导表达,是一种广谱的抗病基因。本研究利用实时定量PCR技术分析转PtDr101基因hpRNA干扰载体的...
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PtDr101基因从三倍体毛白杨(Popu1us tomentosa× P. bolleana)×***]中克隆获得,能够编码NBS-LRR型蛋白,受到水杨酸和甲基茉莉酸诱导表达,是一种广谱的抗病基因。本研究利用实时定量PCR技术分析转PtDr101基因hpRNA干扰载体的表达模式,分析发现4个表达模式显著差异的转基因株系,为进一步功能鉴定提供材料。本研究表明实时定量PCR能够高灵敏度、精确地检测hpRNA干扰情况下的基因表达量。
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