A novel sequence that functions as a promoter element for moderate constitutive expression of transgenes,designated as the PtMCP promoter,was isolated from the woody perennial Populus *** PtMCP promoter was fused to t...
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A novel sequence that functions as a promoter element for moderate constitutive expression of transgenes,designated as the PtMCP promoter,was isolated from the woody perennial Populus *** PtMCP promoter was fused to the GUS reporter gene to characterize its expression pattern in different *** stable Arabidopsis transformants,transcripts of the GUS reporter gene could be detected by RT-PCR in the root,stem,leaf,flower and *** histochemical and fluorometric GUS activity assays demonstrated that the promoter could direct transgene expression in all tissues and organs,including roots,stems,rosette leaves,cauline leaves and flowers of seedlings and maturing *** constitutive expression pattern was similar to that of the CaMV35S promoter,but the level of GUS activity was significantly lower than in CaMV35S promoter::GUS *** also characterized the promoter through transient expression in transgenic tobacco and observed similar expression *** GUS staining and quantitative analysis detected GUS activity in all tissues and organs of tobacco,including roots,stems,leaves,flower buds and flowers,but GUS activity in PtMCP promoter::GUS plants was significantly lower than in CaMV35S promoter::GUS *** results suggested that the PtMCP promoter from poplar is a constitutive promoter with moderate activity and that its function is presumably conserved in different ***,the PtMCP promoter may provide a practical choice to direct moderate level constitutive expression of transgenes and could be a valuable new tool in plant genetic engineering.
Abscisic acid (ABA) is a major plant hormone that plays an important role in responses to abiotic *** ABA-responsive element binding protein/ABRE-binding factor (AREB/ABF) gene subfamily contains crucial transcription...
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Abscisic acid (ABA) is a major plant hormone that plays an important role in responses to abiotic *** ABA-responsive element binding protein/ABRE-binding factor (AREB/ABF) gene subfamily contains crucial transcription factors in the ABA-mediated signaling *** this study, a total of 14 putative AREB/ABF members were identified in the Populus trichocarpa genome using five AREB/ABF amino acid sequences from Arabidopsis thaliana as *** 14 putative Populus subfamily members showed high protein similarities, especially in the basic leucine zipper (bZIP) domain region.A neighbor-joining analysis combined with gene structure data revealed homology among the 14 *** expression patterns of the Populus AREB/ABF subfamily suggested that the most abundant transcripts of 11 genes occurred in leaf tissues, while two genes were most transcribed in root ***, eight Populus AREB/ABF gene members were upregulated after treatment with 100 μM exogenous ABA, while the other six members were *** identified the expression profiles of the subfamily members in Populus tissues and elucidated different response patterns of Populus AREB/ABF members to ABA *** study provided insight into the roles of Populus AREB/ABF homologues in plant response to abiotic stresses.
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