A novel sequence that functions as a promoter element for moderate constitutive expression of transgenes, designated as the PtMCP promoter, was isolated from the woody perennial Populus *** PtMCP promoter was fused to...
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A novel sequence that functions as a promoter element for moderate constitutive expression of transgenes, designated as the PtMCP promoter, was isolated from the woody perennial Populus *** PtMCP promoter was fused to the GUS reporter gene to characterize its expression pattern in different *** stable Arabidopsis transformants, transcripts of the GUS reporter gene could be detected by RT-PCR in the root, stem, leaf, flower and *** histochemical and fluorometric GUS activity assays demonstrated that the promoter could direct transgene expression in all tissues and organs, including roots, stems, rosette leaves, cauline leaves and flowers of seedlings and maturing *** constitutive expression pattern was similar to that of the CaMV35S promoter,but the level of GUS activity was significantly lower than in CaMV35S promoter∷GUS *** also characterized the promoter through transient expression in transgenic tobacco and observed similar expression *** GUS staining and quantitative analysis detected GUS activity in all tissues and organs of tobacco, including roots, stems, leaves, flower buds and flowers, but GUS activity in PtMCP promoter∷ GUS plants was significantly lower than in CaMV35 S promoter∷ GUS *** results suggested that the PtMCP promoter from poplar is a constitutive promoter with moderate activity and that its function is presumably conserved in different ***, the PtMCP promoter may provide a practical choice to direct moderate level constitutive expression of transgenes and could be a valuable new tool in plant genetic engineering.
A novel sequence that functions as a promoter element for moderate constitutive expression of transgenes,designated as the PtMCP promoter,was isolated from the woody perennial Populus *** PtMCP promoter was fused to t...
详细信息
A novel sequence that functions as a promoter element for moderate constitutive expression of transgenes,designated as the PtMCP promoter,was isolated from the woody perennial Populus *** PtMCP promoter was fused to the GUS reporter gene to characterize its expression pattern in different *** stable Arabidopsis transformants,transcripts of the GUS reporter gene could be detected by RT-PCR in the root,stem,leaf,flower and *** histochemical and fluorometric GUS activity assays demonstrated that the promoter could direct transgene expression in all tissues and organs,including roots,stems,rosette leaves,cauline leaves and flowers of seedlings and maturing *** constitutive expression pattern was similar to that of the CaMV35S promoter,but the level of GUS activity was significantly lower than in CaMV35S promoter::GUS *** also characterized the promoter through transient expression in transgenic tobacco and observed similar expression *** GUS staining and quantitative analysis detected GUS activity in all tissues and organs of tobacco,including roots,stems,leaves,flower buds and flowers,but GUS activity in PtMCP promoter::GUS plants was significantly lower than in CaMV35S promoter::GUS *** results suggested that the PtMCP promoter from poplar is a constitutive promoter with moderate activity and that its function is presumably conserved in different ***,the PtMCP promoter may provide a practical choice to direct moderate level constitutive expression of transgenes and could be a valuable new tool in plant genetic engineering.
Abscisic acid (ABA) is a major plant hormone that plays an important role in responses to abiotic *** ABA-responsive element binding protein/ABRE-binding factor (AREB/ABF) gene subfamily contains crucial transcription...
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Abscisic acid (ABA) is a major plant hormone that plays an important role in responses to abiotic *** ABA-responsive element binding protein/ABRE-binding factor (AREB/ABF) gene subfamily contains crucial transcription factors in the ABA-mediated signaling *** this study, a total of 14 putative AREB/ABF members were identified in the Populus trichocarpa genome using five AREB/ABF amino acid sequences from Arabidopsis thaliana as *** 14 putative Populus subfamily members showed high protein similarities, especially in the basic leucine zipper (bZIP) domain region.A neighbor-joining analysis combined with gene structure data revealed homology among the 14 *** expression patterns of the Populus AREB/ABF subfamily suggested that the most abundant transcripts of 11 genes occurred in leaf tissues, while two genes were most transcribed in root ***, eight Populus AREB/ABF gene members were upregulated after treatment with 100 μM exogenous ABA, while the other six members were *** identified the expression profiles of the subfamily members in Populus tissues and elucidated different response patterns of Populus AREB/ABF members to ABA *** study provided insight into the roles of Populus AREB/ABF homologues in plant response to abiotic stresses.
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