Abscisic acid (ABA) is a major plant hormone that plays an important role in responses to abiotic *** ABA-responsive element binding protein/ABRE-binding factor (AREB/ABF) gene subfamily contains crucial transcription...
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Abscisic acid (ABA) is a major plant hormone that plays an important role in responses to abiotic *** ABA-responsive element binding protein/ABRE-binding factor (AREB/ABF) gene subfamily contains crucial transcription factors in the ABA-mediated signaling *** this study, a total of 14 putative AREB/ABF members were identified in the Populus trichocarpa genome using five AREB/ABF amino acid sequences from Arabidopsis thaliana as *** 14 putative Populus subfamily members showed high protein similarities, especially in the basic leucine zipper (bZIP) domain region.A neighbor-joining analysis combined with gene structure data revealed homology among the 14 *** expression patterns of the Populus AREB/ABF subfamily suggested that the most abundant transcripts of 11 genes occurred in leaf tissues, while two genes were most transcribed in root ***, eight Populus AREB/ABF gene members were upregulated after treatment with 100 μM exogenous ABA, while the other six members were *** identified the expression profiles of the subfamily members in Populus tissues and elucidated different response patterns of Populus AREB/ABF members to ABA *** study provided insight into the roles of Populus AREB/ABF homologues in plant response to abiotic stresses.
TRAP法是一种常用的测定端粒酶活性的方法,但并不太适合测定木本植物的端粒酶活性.因此以油松针叶为实验材料,对TRAP法进行改进.参照前人的研究通过对比实验证明,在端粒酶提取过程中将PEG8000加入上清液,可获得高效的端粒酶.在此基础上,对PCR先导引选择TS、模板浓度选择100 ng,最终获得油松针叶高质量的端粒酶PCR产物.应用SYBR Green I替代银染液对电泳凝胶进行染色,获得条带清晰、重复性好的电泳结果.通过对油松针叶、银杏叶片、胡杨愈伤组织、沙冬青悬浮细胞等4种木本植物实验材料进行测定,证明该改进的技术体系可能是适合于木本植物端粒酶活性测定的稳定的、灵敏度高的可行方法.
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