Aim To investigate the mechanism of anti-CD132 monoclonal antibodies (mAbs)inhibiting T cells proliferation in vitro, and their potential values for clinical use. MethodsBALB/c and C57BL/6 mice splenocytes were harves...
详细信息
Aim To investigate the mechanism of anti-CD132 monoclonal antibodies (mAbs)inhibiting T cells proliferation in vitro, and their potential values for clinical use. MethodsBALB/c and C57BL/6 mice splenocytes were harvested for two-ways mixed lymphocyte culture (MLC).Anti-CD132 mAbs (final concentration 100 mg·L^(-1)) were added in MLC on day 0 (group 1) or day 3(group 2). Fluorescence activated cell sorting (FACS) was used to measure the proliferation(carboxy-fluorescein dia cetate, succinimidyl ester, CFSE), apoptosis of T cells (PE-CD3,FTTC-annexin-v), and cell cycle (pro-pidium iodide stain) . The expression of survivin in T cellswas detected by immunochemical stai-ning. Re-sults Multi-generation CFSE-labeled splenocytes werefound dividing and their fluorescent strength decreased in MLC. There was no noticeable change influorescent intensity in group 1 and group 2. On day 3, apoptosis induced by anti-CD132 mAbs wasdetected in part of T cells, but was not detected in the former two days in group 1. In group 2, thenumber of cells in M phase (activated T cells) decreased and apoptot-ic cells increased on day *** phenomena were not observed in control group (P < 0.01). Expression of survivin in T cells wasdetected in control group but not in groups 1 and 2. Conclusion Blockade of CD132 signaling pathwayinhibits T cell proliferation in vitro by means of inducing activated alloreactive T cell apoptosisbut not the resting T cells. Anti-CD132 mAbs may be candidates for clinical applications.
Banff移植病理学会议（Banff Conference on Allograft Pathology，简称Banff会议）是国际最高水平的移植病理学研讨会。首届会议于1991年在加拿大的Banff国家公园举行，遂以此命名[1]。Banff会议经研讨建立了Banff移植肾活组织检查（...
详细信息
Banff移植病理学会议（Banff Conference on Allograft Pathology，简称Banff会议）是国际最高水平的移植病理学研讨会。首届会议于1991年在加拿大的Banff国家公园举行，遂以此命名[1]。Banff会议经研讨建立了Banff移植肾活组织检查（以下简称活检）病理学诊断标准并得到国际各移植中心的广泛采用，随后逐渐建立了移植肝、移植心脏、移植胰腺、移植小肠和复合组织移植等活检诊断标准。
暂无评论