目的:To determine the antiviral activity of rosmarinic acid (RA) by detecting the effect of RA on hepatitis E virus in vitro. The mechanism of the interaction between RA and hepatocytes and virus was studied from the ...
详细信息
目的:To determine the antiviral activity of rosmarinic acid (RA) by detecting the effect of RA on hepatitis E virus in vitro. The mechanism of the interaction between RA and hepatocytes and virus was studied from the cellular and molecular levels by molecular biology test method.方法:MTT was used to detect the toxic effects of water-soluble RA on HepG2, the titer of hepatitis E virus nucleic acid was quantitatively amplified by real-time fluorescence quantitative PCR. The best virus infection (MOI), And then study the effect of drug antiviral effect.结果:0～400μM RA had no toxic effect on HepG2. RA had obvious effect on preventing virus infection and inhibiting the proliferation of virus. However, the preventive effect was not significantly correlated with the concentration of drug *** the effect of RA on the proliferation of the virus was enhanced. When the concentration was 200μM, the inhibitory effect was the best. The expression level of the virus was 1/6 of the positive control group.结论:It is speculated that RA can replace traditional antiviral drugs as an effective therapeutic medicine.
暂无评论