A high selective,sensitive and simple high-performance liquid chromatography（HPLC）method with photodiode array detection（DAD）and mass spectrometric detection has been developed and validated for the simultaneous d...
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A high selective,sensitive and simple high-performance liquid chromatography（HPLC）method with photodiode array detection（DAD）and mass spectrometric detection has been developed and validated for the simultaneous determination,separation and quantification of12 nucleosides and bases,including adenine,cytidine,hypoxanthine,xanthine,uridine,thymine,deoxythymine,guanosine,deoxyguanosine,inosine,deoxyinosine and thymidine in Ruditapes philippinarum,Meretrix meretrix Linnaeus,Cyclina sinensis,and Mactra veneriformis *** separation was performed on a Waters XSelect HSS Ccolumn（4.6×250 mm,5urn）with a gradient elution at a flow rate of 0.6 rnl/*** diode-array detector wavelength was set at 254nm for the UV detection of nucleosides and *** separation of these compounds was obtained in less than 40 *** calibration curves showed good linearity（r>0.9995 for all the investigated analytes）,satisfactory precision（RSD<1.66）and good recovery（from 97.86 to 101.83）within the test *** presence of each nucleoside in samples was ascertained by comparison of MS data,UV spectra and retention time with *** established method was successfully applied to simultaneous determination of twelve nucleosides and nucleobases in 48 batches of samples from different species collected from January to *** results showed that the harvest months in the total nucleoside and nucleobase contents reached the highest were July for Ruditapes philippinarum（4160.47ug/ml）,June for Meretrix meretrix Linnaeus（2450.56ug/ml）,July for Cyclina sinensis（2587.10ug/ml）,and July for Mactra veneriformis Reeve（2850.91ug/ml）,*** study could be used to control the quality of Ruditapes philippinarum,Meretrix meretrix Linnaeus,Cyclina sinensis,and Mactra veneriformis Reeve in the future.
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