According to the data concerned and with the aids of DNA star software,the 250-460 amino acids of the glycoprotein B of Marek’s disease virus GA strain was selected as target fragment for tandem *** pairs of primers ...
详细信息
According to the data concerned and with the aids of DNA star software,the 250-460 amino acids of the glycoprotein B of Marek’s disease virus GA strain was selected as target fragment for tandem *** pairs of primers were designed for amplifying the upstream fragment gB(U) and the downstream fragment gB(L/D).And an ATG GCG was added to the 5’ terminus of gB(U),a TAA was added to the 3’ terminus of gB(L/D) and a linker of nine amino acids was designed at the 5’ terminus of gB(L/D).Thus a complete ORF formed when gB(U) contacted with gB(L/D).PCR products were cloned by general molecular clone method.A transfer plasmid vector pEFMDgB(U/L/D) was constructed and then cotransfected with fowl poxvirus in CEF by calcium phosphate method and the recombinant virus was selected with MPA and its specificity was identified positive in infected *** result showed that the tandem expressing products have immunocompetence,and this will bring a prospect for developing a candidate MD vaccine.
暂无评论