Two pairs of primers were designed according to the N gene sequence of PEDV-CV777 strain in Genebank (No. AF353511). PCR amplification product of outer-primers was 1328bp, and the inter-primers amplification product w...
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Two pairs of primers were designed according to the N gene sequence of PEDV-CV777 strain in Genebank (No. AF353511). PCR amplification product of outer-primers was 1328bp, and the inter-primers amplification product was 538bp. With the primers, a nested PCR assay was established to detect PEDV. This method was sensitive and specific and could be used in PEDV diagnosis and epidemiological investigation.
The purpose of the present study was to determine the prevalence of swine Hepatitis E virus (HEV) infection in Xinjiang. 813 swine serum samples collected from 1 to 12 months of age at 9 swine farms in Xinjiang region...
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The purpose of the present study was to determine the prevalence of swine Hepatitis E virus (HEV) infection in Xinjiang. 813 swine serum samples collected from 1 to 12 months of age at 9 swine farms in Xinjiang region were tested by ELISA for the presence of IgG antibodies against HEV. The recombinant protein pUS166 containing region 452-617aa of the ORF2 of HEV US strain was used as coating antigen. The result showed that anti –HEV IgG were detected in 265 of 405 pigs (65.43%) in one group and 238 of 408 pigs (58.33%) in another group , and that the seropositivity rate was not related to geographic district and breeds, but differed remarkably by age, being 40% among the 1- to 3-month-old piglets, but 77.33% among ones over 3-month-old. It suggested that swine HEV was widespread in different geographic regions of XinJiang.
Using a pair of specific primers designed according to the relevant nucleotide sequence of Porcine Reproductive and Respiratory Syndrome Virus(PRRSV)VL2332 strain, Lv strain and Classical Swine Fever Virus(CSFV) C str...
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Using a pair of specific primers designed according to the relevant nucleotide sequence of Porcine Reproductive and Respiratory Syndrome Virus(PRRSV)VL2332 strain, Lv strain and Classical Swine Fever Virus(CSFV) C strain from GenBank, RT-PCR method was established to detect these two viruses at the same time. With this method, 87 tissue samples of 24 pig farms from Jiangsu, Shanghai and Fujian were tested. Among these samples 75 were PRRS positive, 56 were CSF positive, 53 were both PRRS and CSF positive, which amounts to 60.9 percent of all samples. Combined with regional epidemiology analysis, We concluded that samples from Fujian mainly mixed infected with PRRSV and CSFV, but samples from Jiansu simply infected with PRRSV.
Five isolates of pigeon paramyxovirus Ⅰ（PPMV-Ⅰ）,designated as YZ9712,NC9701,NP9904,PB9601 and SZa9803,were isolated from pigeons during epizootics recently in *** F gene functional domains were amplified by RT-PCR...
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Five isolates of pigeon paramyxovirus Ⅰ（PPMV-Ⅰ）,designated as YZ9712,NC9701,NP9904,PB9601 and SZa9803,were isolated from pigeons during epizootics recently in *** F gene functional domains were amplified by RT-PCR and then cloned into pGEMDR○-T vector and sequenced.A phylogenetic tree based on nucleotide sequence of the F gene segment revealed all the strains were divided into seven genotypes,and five PPMV-Ⅰ isolates similar to strains 1168/84 and 760/83 were grouped into subgenotype Ⅵ*** homology between these five isolates were 98.3%～99.4% and 97.8%～99.4% at the nucleotide and amino acid levels *** deduced amino acid sequence at the cleavage site of the F protein was of virulent strains112K-R-Q-K-R-F117?All the isolates had highly genetic similarity to the England reference strain 1168/84 and Wales reference strain 760/83 of PPMV-Ⅰ,suggesting that they might have originated from a common ancestor.
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