Five isolates of pigeon paramyxovirus Ⅰ（PPMV-Ⅰ）,designated as YZ9712,NC9701,NP9904,PB9601 and SZa9803,were isolated from pigeons during epizootics recently in *** F gene functional domains were amplified by RT-PCR...
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Five isolates of pigeon paramyxovirus Ⅰ（PPMV-Ⅰ）,designated as YZ9712,NC9701,NP9904,PB9601 and SZa9803,were isolated from pigeons during epizootics recently in *** F gene functional domains were amplified by RT-PCR and then cloned into pGEMDR○-T vector and sequenced.A phylogenetic tree based on nucleotide sequence of the F gene segment revealed all the strains were divided into seven genotypes,and five PPMV-Ⅰ isolates similar to strains 1168/84 and 760/83 were grouped into subgenotype Ⅵ*** homology between these five isolates were 98.3%～99.4% and 97.8%～99.4% at the nucleotide and amino acid levels *** deduced amino acid sequence at the cleavage site of the F protein was of virulent strains112K-R-Q-K-R-F117?All the isolates had highly genetic similarity to the England reference strain 1168/84 and Wales reference strain 760/83 of PPMV-Ⅰ,suggesting that they might have originated from a common ancestor.
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