目的:探索射频消融(radiofrequency ablation,RFA)干预下肝癌细胞凋亡及周围肝组织病理学变化规律,为在临床治疗中实现病理性完全消融(pathological complete ablation,PCA)提供病理学基础.方法:采用肿瘤细胞悬液肝内注入法构建兔肝VX2肿瘤模型,在直视定位下行肝VX2肿瘤单电极射频消融术,由消融中心依次逐层取材,采用HE染色观察肿瘤以及周围肝组织各个类型细胞的病理学变化,同时应用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记(TdT mediated-dUTP nick end labeling)法检测各部位细胞凋亡情况.结果:RFA术后,以电极为中心的周围肿瘤组织依次出现凝固性坏死区、坏死与细胞凋亡混合区、细胞凋亡区.其中凝固性坏死区、坏死与细胞凋亡混合区可见大量炎细胞浸润,单纯细胞凋亡区未见炎性细胞浸润.在坏死与细胞凋亡混合区、细胞凋亡区域可见残余存活的肝癌细胞;在肿瘤周围的肝细胞有部分坏死肝细胞,但以细胞凋亡为主,中央静脉、汇管区血管内皮细胞以及肝内小胆管上皮可见部分肝细胞凋亡.结论:通过对兔肝脏VX2肿瘤射频消融后的病理表现,为临床治疗中进一步提高射频消融清除癌灶的能力和效率提供理论基础.
Objective To propose a revised definition of allergy and a novel procedure for applying allergy *** The related studies and theories were reviewed and analyzed, and the problem in defining ALLERGY was *** and Conclusi...
Objective To propose a revised definition of allergy and a novel procedure for applying allergy *** The related studies and theories were reviewed and analyzed, and the problem in defining ALLERGY was *** and Conclusions Allergic diseases should be defined as a group of mast cell or basophil activation provoked non-infection diseases, including IgE mediated, IgG mediated and non-lgE or non-lgG mediated (non-immunological) *** upon the proposed definition above, the ideal laboratory examination procedure for allergy should be as follows: Skin prick and skin patch tests (highly suspicious of allergy)(@)allergen-induced basophil activation test (confirmation of allergy)(@) slgE and slgG measurement (confirmation of subtype of allergy)(@)final diagnosis: IgE, IgG or non-immunological allergy.
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