银杏叶提取物(Extracts of Ginkgo biloba,EGb)具有诸多生物活性,但银杏酚酸的存在限制了EGb的应用。目前,去除EGb中银杏酚酸的方法主要有有机溶剂法和柱层析法,笔者在有机溶剂法的基础上提出配位-有机溶剂法,即在有机溶剂法最佳工艺条...
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银杏叶提取物(Extracts of Ginkgo biloba,EGb)具有诸多生物活性,但银杏酚酸的存在限制了EGb的应用。目前,去除EGb中银杏酚酸的方法主要有有机溶剂法和柱层析法,笔者在有机溶剂法的基础上提出配位-有机溶剂法,即在有机溶剂法最佳工艺条件的基础上引入金属离子配位以增加去除银杏酚酸的效果。结果表明:利用配位-有机溶剂法可以有效去除银杏酚酸,并有效减少有机溶剂洗涤次数,且不影响总银杏黄酮苷和总银杏内酯的含量;同时,EGb中的部分黄酮与锌离子形成配合物,可以提高EGb的抗肝癌细胞(HepG2)的活性。
Subfamily 2 of SNF1-related protein kinase(SnRK2) plays important roles in plant abiotic stress responses as a global positive regulator of abscisic acid *** the genome of the model tree Populus trichocarpa,12 SnRK2 g...
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Subfamily 2 of SNF1-related protein kinase(SnRK2) plays important roles in plant abiotic stress responses as a global positive regulator of abscisic acid *** the genome of the model tree Populus trichocarpa,12 SnRK2 genes have been identified,and some are upregulated by abiotic *** this study,we heterologously overexpressed the PtSnRK2 genes in Arabidopsis thaliana and found that overexpression of PtSnRK2.5 and PtSnRK2.7 genes enhanced stress *** the PtSnRK2.5 and PtSnRK2.7 overexpressors,chlorophyll content,and root elongation were maintained under salt stress conditions,leading to higher survival rates under salt stress compared with those in the wild *** analysis revealed that PtSnRK2.7 overexpression affected stress-related metabolic genes,including lipid metabolism and flavonoid metabolism,even under normal growth ***,the stress response genes reported to be upregulated in Arabidopsis SRK2 C/SnRK2.6 and wheat SnRK2.8 overexpressors were not changed by PtSnRK2.7 ***,PtSnRK2.7 overexpression widely and largely influenced the transcriptome in response to salt stress;genes related to transport activity,including anion transport-related genes,were characteristically upregulated,and a variety of metabolic genes were specifically *** also found that the salt stress response genes were greatly upregulated in the PtSnRK2.7 *** together,poplar subclass 2 PtSnRK2 genes can modulate salt stress tolerance in Arabidopsis,through the activation of cellular signaling pathways in a different manner from that by herbal subclass 2 SnRK2 genes.
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