Winter lignified stems of Bauhinia galpinii were used as explants for tissue culture,and the effects of different hormones on inductivity rate and rooting rate were investigated by supplementing different *** results ...
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Winter lignified stems of Bauhinia galpinii were used as explants for tissue culture,and the effects of different hormones on inductivity rate and rooting rate were investigated by supplementing different *** results indicated that buds were induced from a 2 cm high young plantlet cultured in MS medium supplemented with 1.0 mg·L-1 6-BA and 0.1 mg·L-1 NAA for 20 d,and the induction rate was 100%.The mean amount of buds was *** medium of MS+ 0.4mg·L-1 NAA was used for rooting and the rooting rate was 85%.The experiment strategy to establish tissue culture system from lignified stem was feasible,which could be used for cold acclimation and cold resistant mutant selection.
A total of 1,160 differentially expressed genes induced by Marssonina brunnea f. sp. muhigermtubi were identified in Populus deltoides cv. ＇Lux＇ （1-69/55） with two-colour cDNA microarray including 2,952 cDNAs from...
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A total of 1,160 differentially expressed genes induced by Marssonina brunnea f. sp. muhigermtubi were identified in Populus deltoides cv. ＇Lux＇ （1-69/55） with two-colour cDNA microarray including 2,952 cDNAs from two cDNA libraries constructed with 72 h inoculated poplar leaves. Functional analysis showed that 1,160 genes were classified into 11 functional categories that are involved in metabolism （15.9%）, signal transduction （9.5%）, transcription and replication （8.7%）, and cell rescue and defense （7.8%）. Among them, 926 genes were sporadically localized on 19 linkage groups. Chromosome 2 contained 102 （11%） differentially expressed genes, followed by chromosome 1 which contains 93 genes （10%）, and chromosome 17 had the least number of differentially expressed genes. Clustering of expressed sequence tags （ESTs） in poplar genome was observed at the terminal regions of several chromosomes. The relationship between cluster of genes and plant defense response would be further studied.
Pinus massoniana L. is one of the important trees for afforestation in South China. The efficient system of plant regeneration from mature zygotic embryos and seedlings of masson pine was established in this study. Th...
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Pinus massoniana L. is one of the important trees for afforestation in South China. The efficient system of plant regeneration from mature zygotic embryos and seedlings of masson pine was established in this study. The influences of basal media, hormones and methods for buds induction, shoots elongation and rooting were studied. The results indicate that DCR medium with 0.5 mg/L BA and 0.05 mg/L IBA shows the highest differentiation rate of adventitious buds. Induction and multiplication of axillary buds take aseptic seedlings as explants. KT has better effect than BA on the axillary buds induction. The best axillary buds induction medium is DCR medium supplemented with 1 mg/L KT and 0.2 mg/L IBA. After culturing on GD medium with 0.1 mg/L BA and 0.2 mg/L IBA for elongation,the buds were transferred on the 1/2 GD medium with 2 mg/L IBA and 0.05 mg/L BA for adventitious roots induction. Paraffin slice indicates that the adventitious buds developed from the meristematic tissue of cotyledonary epicuticula.
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