Winter lignified stems of Bauhinia galpinii were used as explants for tissue culture,and the effects of different hormones on inductivity rate and rooting rate were investigated by supplementing different *** results ...
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Winter lignified stems of Bauhinia galpinii were used as explants for tissue culture,and the effects of different hormones on inductivity rate and rooting rate were investigated by supplementing different *** results indicated that buds were induced from a 2 cm high young plantlet cultured in MS medium supplemented with 1.0 mg·L-1 6-BA and 0.1 mg·L-1 NAA for 20 d,and the induction rate was 100%.The mean amount of buds was *** medium of MS+ 0.4mg·L-1 NAA was used for rooting and the rooting rate was 85%.The experiment strategy to establish tissue culture system from lignified stem was feasible,which could be used for cold acclimation and cold resistant mutant selection.
A total of 1,160 differentially expressed genes induced by Marssonina brunnea f. sp. muhigermtubi were identified in Populus deltoides cv. ＇Lux＇ （1-69/55） with two-colour cDNA microarray including 2,952 cDNAs from...
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A total of 1,160 differentially expressed genes induced by Marssonina brunnea f. sp. muhigermtubi were identified in Populus deltoides cv. ＇Lux＇ （1-69/55） with two-colour cDNA microarray including 2,952 cDNAs from two cDNA libraries constructed with 72 h inoculated poplar leaves. Functional analysis showed that 1,160 genes were classified into 11 functional categories that are involved in metabolism （15.9%）, signal transduction （9.5%）, transcription and replication （8.7%）, and cell rescue and defense （7.8%）. Among them, 926 genes were sporadically localized on 19 linkage groups. Chromosome 2 contained 102 （11%） differentially expressed genes, followed by chromosome 1 which contains 93 genes （10%）, and chromosome 17 had the least number of differentially expressed genes. Clustering of expressed sequence tags （ESTs） in poplar genome was observed at the terminal regions of several chromosomes. The relationship between cluster of genes and plant defense response would be further studied.
利用PCR扩增产物直接测序的方法分析广义青篱竹属 (Arundinaria)中有关争议类群的代表种或模式种 (毛竹为外类群 )等 18种竹种的核糖体DNA内转录间隔区 (InternalTranscribedSpacers ,ITS)序列。通过最简约性分析产生的ITS系统发育树表明 ,供试竹种形成一个自然的单系类群 ,这说明广义青篱竹属中这些不同的类群归属青篱竹属是合理的。 17种竹种可聚为 2大分支 :其中斑苦竹 (A .oleosa)、仙居苦竹 (A .hsienchuensis)、茶秆竹 (A .amabilis)、长叶苦竹 (A .chino)、苦竹 (A .amara)、宜兴苦竹 (A .yixingensis)、菲白竹 (A .fortunei)、翠竹 (A .pygmaea)为一个分支 ;而大明竹 (A .graminea)、巴山木竹 (A .fargesii)、冷箭竹 (A .faberi)、凤竹 (A .hupehense)、鼓节矢竹 (***)、矢竹 (Pseudosasajaponica)、短穗竹 (Brachystachyumdensiflorum)、肿节竹 (A .oedogonata)、少穗竹 (A .sulcata)组合在另一分支。ITS系统发育树还表明 ,大明竹与巴山木竹、鼓节矢竹与矢竹、少穗竹与短穗竹和肿节竹关系极为密切 ,均得到较高的Bootstrap(分别为 99%、10 0 %和 87% )的支持 ;茶秆竹与仙居苦竹关系非常密切 ,茶秆竹可归隶到青篱竹属中 ;翠竹和菲白竹关系密切 。
杨树是重要的速生用材树种之一,采用基因工程技术开展转基因杨树的研究,是培育抗逆、抗虫和抗病杨树新品种的有效途径。本研究以新疆杨(Populus alba ***)为材料,开展了新疆杨叶盘植株再生及对遗传转化受体系统的影响因素的研究。探讨...
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杨树是重要的速生用材树种之一,采用基因工程技术开展转基因杨树的研究,是培育抗逆、抗虫和抗病杨树新品种的有效途径。本研究以新疆杨(Populus alba ***)为材料,开展了新疆杨叶盘植株再生及对遗传转化受体系统的影响因素的研究。探讨了基本培养基、生长素、细胞分裂素、叶盘接种方式、抗生素等因素对新疆杨叶盘再生不定芽的影响。结果表明:生长素的种类与浓度对芽的分化频率影响不显著;不同种类的细胞分裂素影响芽分化频
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