Winter lignified stems of Bauhinia galpinii were used as explants for tissue culture,and the effects of different hormones on inductivity rate and rooting rate were investigated by supplementing different *** results ...
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Winter lignified stems of Bauhinia galpinii were used as explants for tissue culture,and the effects of different hormones on inductivity rate and rooting rate were investigated by supplementing different *** results indicated that buds were induced from a 2 cm high young plantlet cultured in MS medium supplemented with 1.0 mg·L-1 6-BA and 0.1 mg·L-1 NAA for 20 d,and the induction rate was 100%.The mean amount of buds was *** medium of MS+ 0.4mg·L-1 NAA was used for rooting and the rooting rate was 85%.The experiment strategy to establish tissue culture system from lignified stem was feasible,which could be used for cold acclimation and cold resistant mutant selection.
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