Thirty superior trees of Chinese fir from eight provinces were analyzed on genetic variation with RAPD method.A total of 284 bands,including 190 polymorphic ones,were produced with 26 primers, and mean genetic distanc...
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Thirty superior trees of Chinese fir from eight provinces were analyzed on genetic variation with RAPD method.A total of 284 bands,including 190 polymorphic ones,were produced with 26 primers, and mean genetic distance between the thirty superior trees was 0.4909,indicating a high level of genetic diversity among the tress *** superior trees,coded as ZJZ 01 and JXS26,showed a high value in genetic similarity,which meant that only one of them could be essentially preserved in gene *** clustering analysis indicated that most of the superior trees could be clustered at a relatively high genetic distance and rich genetic variation could be expected in the superior tree *** might be valuable for marker-assisted germplasm collection,parent grouping in hybridization and genetic diversity evaluation on breeding materials in improvement of Chinese fir.
ITS sequences of 15 representative species of five sections in the genus Populus L. were determined. By using direct sequencing of PCR product, it was found that the fragments of internal transcribed spacers （ITS） a...
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ITS sequences of 15 representative species of five sections in the genus Populus L. were determined. By using direct sequencing of PCR product, it was found that the fragments of internal transcribed spacers （ITS） are about 594 bp in length. The length of ITS1 and ITS2 is about 220 bp and 210 bp, respectively, while that of 5.8s is 164 bp. Its G+C content is about 69.0%. The number of phylogenetically informative loci is higher in ITS2 than in ITS1. Transversion and transition are two main factors that drive the ITS evolution, and more insertions and deletions occurred in ITS2. Taking Salix matsudana Koidz. and Salix suchowensis Cheng as outgroups, phylogenetic analysis of ITS sequences using PAUP 4.0 software indicated that Populus is monophyletic group and can be divided into two main clades. One is the section Leuce , and the other is the remaining sections.
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