1植物名称巨紫荆((Cercls gtgantea Cheng et Keng f.),植株来源于本校校园内。2材料类别9月上旬巨紫荆荚果中未成熟种胚。3培养条件幼胚萌发与无菌苗生长的培养基:(1)-(3)1/2MS+6-BA0.5mg.L-1(单位下同)+蔗糖(2%、2...
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1植物名称巨紫荆((Cercls gtgantea Cheng et Keng f.),植株来源于本校校园内。2材料类别9月上旬巨紫荆荚果中未成熟种胚。3培养条件幼胚萌发与无菌苗生长的培养基:(1)-(3)1/2MS+6-BA0.5mg.L-1(单位下同)+蔗糖(2%、2.5%、3%);(4)-(6)1/2MS0+6-BA0.5+蔗;膪(2%、2.5%、3%);(7)~(8)1/2MS+6-BA0.5+维生素C(Vc,0.5、1.0);(9)-(10)1/2MS0+6-BA0.5+Vc(0.5、1.0)。芽增殖培养基:(11)MK+6-BA0.5+NAA0.1;(12)MK+6-BA0,75+NAA0.1;(13)MK+6-BA0.5+NAA0.25;(14)MK+6-BA0.75+NAA0.25;(15)MK+6-BA1.0+NAA0.25;(16)MK+6-BA1.5+NAA0.25;壮苗培养基:(17)MK+6-BA0.75+NAA0.1+1.0g.L-1水解酪蛋白(CH);08)MK+***1.O+NAA0.1+1.0g.L-1CH;(19)MK+6-BA0.75+NAA0.25+1.0g.L-1CH;(20)MK+6-BAl.0+NAAO.25+1.0g.L-1 CH;(21)MK+***1.5+NAA0.25+1.0g.L-1CH。生根培养基:(22)MK+IBA0.5+NAA1.O;(23)MK+IBA1.O+NAA0.5;(24)MK+IBA1.0+NAA1.O;(25)MK+IBA1.5+NAA0.5;(26)MK+IBA1.5+NAA1.0。培养基(7)-(26)添加2.5%的蔗糖。
The development, cytological characters and ultrastructure of phloem ganglion in the nodal region of Phyllostachys edulis (Carr.) H. de Lehaie, a most economically important bamboo, were investigated and the possi...
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The development, cytological characters and ultrastructure of phloem ganglion in the nodal region of Phyllostachys edulis (Carr.) H. de Lehaie, a most economically important bamboo, were investigated and the possible physiological function of this special structure was proposed. The phloem ganglion derived directly from procambium is situated at the sites where the vascular bundle forks and is present in pairs. The phloem ganglion is spindle_like in appearance and usually consists of 4 to 6 layers. Two kinds of cells in the ganglion could be distinguished. In the middle, there are two layers of filiform cells with pointed ends so that there are no normal sieve plates. Nevertheless, there are many pits on the lateral wall of the filiform cells. The other type of cells located at both ends of the spindle which possess an intermediate form between the filiform cell and the normal sieve tube. The walls of these cells towards the filiform cells are strongly convex forming a special sieve plate. Ultrastructure study showed that cells in the ganglion are connected by enriched plasmodesma. During early differentiation, the paramural body and the ingrowth of cell wall could be observed. It indicates that the cells of phloem ganglion have the character of transfer cells. The organelles in the mature cells are mainly plastids with abundant accumulation of proteins of crystalline structure. The above_mentioned results suggest that the physiological function of the phloem ganglion is closely related with substance transport.
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