Embryogenesis of gymnosperm plant is a complex,dynamic and multistage *** this study,the Chinese fir seeds were analyzed at six stages spanning important developmental phases. The proteome profiles of seed samples wer...
详细信息
Embryogenesis of gymnosperm plant is a complex,dynamic and multistage *** this study,the Chinese fir seeds were analyzed at six stages spanning important developmental phases. The proteome profiles of seed samples were analyzed using 2-D difference gel electrophoresis (DIGE).One hundred thirty-six protein spots differing in kinetics of appearance were subjected to LC-MS/MS and MALDI-TOE Mass spectrometry data were interpreted that identified 77 of them. Hierarchical cluster analysis of the 77 spots recognized three main developmental profiles. Functional annotation of the Chinese fir developing seed proteins revealed that PCD is a central process for seed development,which may be multiple programs to execute PCD process in Chinese fir *** were also identified involved in carbon metabolism,Met metabolism,energy production,protein storage,synthesis and stabilization,disease/defense,cytoskeleton protein and proteins related to embryo *** involved in carbon metabolism were higher expression at the early stages,which probably provide substrates for starch *** related to Met metabolism were accumulated at the later stages,which had key role in the building block for protein and polyamines and hormone ethylene and implicated seed development tending to mature. This study provides a reference map for seed development of Chinese fir and might serve as potential markers for specific stage of seed development.
暂无评论