目的构建并鉴定SETD4基因敲除小鼠,为研究SETD4的生物学功能提供动物模型。方法将引进的SETD4flox/+小鼠与EIIa-Cre小鼠进行杂交繁殖,得到基因型为SETD4+/-.EIIa-Cre的小鼠;再与C57BL/6小鼠杂交去除Cre酶,获得杂合子SETD4+/-小鼠;该小鼠自交获得纯合子SETD4-/-小鼠。通过PCR法鉴定子代小鼠的基因型;RT-PCR、荧光定量PCR方法鉴定纯合子的SETD4基因敲除小鼠SETD4m RNA表达情况;HE染色观察小鼠肝、肺组织的形态学变化。结果 PCR结果表明子代小鼠的基因型符合SETD4-/-;纯合子基因敲除小鼠SETD4 m RNA水平显著低于野生型小鼠;SETD4基因敲除小鼠肝、肺组织的形态学特征与野生型小鼠相比无明显差异。结论本研究基于Cre/loxp系统,成功构建并鉴定了SETD4基因敲除小鼠。
Sepsis is systemic inflammatory response syndrome(SIRS)associated with the presence of pathogenic microorganisms or their ***(LPS)is an important component of the outer membrane of Gram negative bacteria and has a piv...
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Sepsis is systemic inflammatory response syndrome(SIRS)associated with the presence of pathogenic microorganisms or their ***(LPS)is an important component of the outer membrane of Gram negative bacteria and has a pivotal role in inducing Gram negative *** play an essential role in infection and *** recognition of LPS is of particular importance in the defense system and pattern recognition receptors(PRR)have been considered to be important in initial steps for cellular recognition of LPS and consequence initiation of LPS *** the past few years,intense research in the fields of PRR and their recognition mechanism has been *** this review,we attempt to expound recent research advances of macrophage PRRs for LPS recognition and their mechanism.
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