Biapenem, a new parenteral carbapenem, has been widely used for treating bacterial infections. A simple, effective and accurate method based on solid-phase extraction （SPE） and HPLC was developed for the quantitativ...
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Biapenem, a new parenteral carbapenem, has been widely used for treating bacterial infections. A simple, effective and accurate method based on solid-phase extraction （SPE） and HPLC was developed for the quantitative determination of biapenem in human plasma. Stability and feasibility of the method was validated through a series of experiments. Using Vitamin B6 as an internal standard, analyte was separated on a Capcell Pak C18 column after SPE on Oasis hydrophilic-lipophilic balance （HLB） cartridge. The mobile phase was comprised of 0.05 mol/L NaH2PO4 （pH 5.7） and methanol （98：2, v/v） at a flow rate of 1.0 mL/min. Ultraviolet absorbance was measured at 300 nm. The calibration curve was linear in the concentration range of 0.04-50.00 μg/mL, and the lower limit of quantification was as low as 0.04 μg/mL. Recovery rates of biapenem at 0.10, 5.00, and 25.00 μg/mL were about 70%. The validated method has been successfully applied for quantifying biapenem in human samples and a pharmacokinetic study of 12 healthy volunteers who received three different doses （150, 300 and 600 mg） of biapenem by intravenous infusion. Our method has featured good accuracy and precision, and the processed sample was stable. Therefore, it can be propagated for clinical use.
目的:晚期糖化终产物受体(receptor for advancedglycation end-products,RAGE)在糖尿病慢性并发症中起着重要作用。RAGE通过与其配体包括晚期糖化终产物(AGEs)等相互作用,诱导氧化应激及炎症反应,促使糖尿病慢性并发症包括糖尿病肾病...
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目的:晚期糖化终产物受体(receptor for advancedglycation end-products,RAGE)在糖尿病慢性并发症中起着重要作用。RAGE通过与其配体包括晚期糖化终产物(AGEs)等相互作用,诱导氧化应激及炎症反应,促使糖尿病慢性并发症包括糖尿病肾病的发生与发展。丹参酮ⅡA(TanshinoneⅡA,TanⅡA)是中药丹参的脂溶性有效单体,具有抗氧化应激等作用。本研究拟探讨丹参酮ⅡA对高糖培养人肾系膜细胞RAGE表达和氧化应激水平的影响。
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