PURPOSE:Mesenchymal Stem Cells (MSCs) transplantation provides a novel strategy for treatment of human *** imaging (MRI) could in vivo track the transplanted stem cells labeled with superparamagnetic iron oxide (SPIO)...
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PURPOSE:Mesenchymal Stem Cells (MSCs) transplantation provides a novel strategy for treatment of human *** imaging (MRI) could in vivo track the transplanted stem cells labeled with superparamagnetic iron oxide (SPIO).However,the effect of SPIO upon labeled MSCs is still unclear on a cellular *** this study,the biological characteristics of rat MSCs labeled with SPIO were *** AND METHODS:The MSCs were isolated from the bone marrow of 5 adult Sprague-Dawley rats and labeled with home synthesized SPIO particles at the final iron concentration of 20 μg/*** MSCs was confirmed with Prussian blue staining and transmission electron *** quantity of iron per cell was determined by atomic absorption *** viability,proliferation,membranous antigen and multiple differentiation ability between labeled and unlabeled MSCs were ***:The labeling efficiency of SPIO was about 98% revealed by Prussian blue *** SPIO particles located in the endosomal vesicles of the MSCs were confirmed by a transmission electron *** significant differences were found in cell viability,proliferation,membranous antigen and multiple differentiation ability between labeled and unlabeled MSCs (P>0.05).CONCLUSION:MSCs could be effectively labeled by SPIO particles without influencing its main biological characteristics.
PURPOSE:To compare two inoculation methods in order to find a better modality for establishing VX2 rabbit hepatic *** AND METHODS:Twenty-eight New Zealand White rabbits were randomly divided into two *** inoculations ...
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PURPOSE:To compare two inoculation methods in order to find a better modality for establishing VX2 rabbit hepatic *** AND METHODS:Twenty-eight New Zealand White rabbits were randomly divided into two *** inoculations were performed by open *** Group 1,14 rabbits received freshly prepared VX2 tumour cell suspension in a volume of 0.2 *** tumour cell suspension was injected slowly into the rabbit liver parenchyma using a 18-gauge *** Group 2,0.2 ml suspension of small minced tumor fragments of VX2 carcinoma inserted into a 18-gauge was implanted into the liver parenchyma of 14 *** tumor survival ratio,the tumor size,extrahepatic metastases were evaluated and compared using CT scan,selective angiography and pathological specimens of individual ***:Successful liver tumor growth was achieved in 10 of the 14 rabbits in Group 1 (80%) and 14 of the 14 rabbits in Group 2 (100%) (P <.05).At 2 weeks after implantation,single nodular tumor in the liver was observed in 4 of the 10 rabbits in Group 1 (80%) and 10 of the 14 rabbits in Group 2 (90%) (P <.05),and multinodular tumors in the liver were observed in4 of the 10 rabbits in Group 1 (80%) and 10 of the 14 rabbits in Group 2 (90%) (P <.05),as determined by CT *** hypervascular tumor staining was revealed on selective *** metastases were seen in 5 rabbits in Group 1(40%)and 3 rabbits in Group 2 (20%)(P <.05).Image findings of VX2 hepatic tumor were in concordance with ***:As a simple and effective method for induction of hepatic tumor,direct implantation of VX2 tumour fragment into the liver using fine needle achieved higher success rate than injecting VX2 tumour cell into the *** rabbit hepatic tumors inoculated by this method may be more suitable animal model of investigations of liver cancer therapeutics.
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