小批量制备了电泳纯鲨鱼肝铁蛋白(Liver ferritin of Sphyma zygaena,SZLF).用透射电子显微镜技术研究SZLF的铁核和蛋白壳亚基解离和重组过程.用盐酸解离(pH=1.5)和分离膜透析技术制备铁蛋白亚基,并用酸碱中和方法重组亚基成为脱铁核铁...
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小批量制备了电泳纯鲨鱼肝铁蛋白(Liver ferritin of Sphyma zygaena,SZLF).用透射电子显微镜技术研究SZLF的铁核和蛋白壳亚基解离和重组过程.用盐酸解离(pH=1.5)和分离膜透析技术制备铁蛋白亚基,并用酸碱中和方法重组亚基成为脱铁核铁蛋白(apoSZLF),同时将胰岛素(Insulin,INS)包裹于apoSZLF蛋白壳内,构建纳米INS核-SZLF.用电子光谱、MALDI-TOF质谱和SDS-PAGE技术分别揭示了纳米INS核-SZLF分子结构的真实性,提出铁蛋白亚基包裹INS构建为纳米INS核-铁蛋白的途径.
The biological effects during seed germination were investigated after the dry seeds of Stevia rebaudianum Bertoni were implanted with carbon ion beam of 75 keV and 10 14 ions/cm 2. The results showed that the g...
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The biological effects during seed germination were investigated after the dry seeds of Stevia rebaudianum Bertoni were implanted with carbon ion beam of 75 keV and 10 14 ions/cm 2. The results showed that the germination rate of carbon ion implanted seeds was slightly higher than that of the control, but the survival rate of the treated seedlings, on the contrary, was lower than that of the control (P<0.02), while the height of the treated seedlings was significantly higher than that of the control (P<0.01). On the 4th day after germination, the leaf cell wall in the treated group was thick, some high electron_dense substance deposited in the enlarged plasmodesma; Cell membrane creased with high electron_dense granules deposited on it. The plasma membrane protruded towards cell wall, and the granules shifted via plasmodesma or deposited onto cell wall. These phenomena may be related to the conveyance of implanted ions across cell wall, or be related to the accumulation of callose. In addition, the implantation of carbon ions could increase the lamellae of the chloroplast and cause high development of the chloroplast which sometimes contained two plastid centers in an individual chloroplast. Also, the highly developed cristae, abundant mitochondria and typical crystalloid structure in microbody could be found. All these results indicated that the anabolic and catabolic activities in the seedlings implanted with carbon ions before germination were obviously more active than those in the controls.
选用柱层析、电泳和反相高效液相色谱（RP-HPLC）技术制备质谱纯棕色固氮菌细菌铁蛋白（Bacteri-al ferritin ofAzotobacter vinelandii,AVBF）,并采用释放铁动力学和肽质量指纹图谱（Peptide mass fingerprint-ing,PMF）技术分别鉴定A...
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选用柱层析、电泳和反相高效液相色谱（RP-HPLC）技术制备质谱纯棕色固氮菌细菌铁蛋白（Bacteri-al ferritin ofAzotobacter vinelandii,AVBF）,并采用释放铁动力学和肽质量指纹图谱（Peptide mass fingerprint-ing,PMF）技术分别鉴定AVBF。基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）和电泳技术揭示AVBF亚基之间相互作用强度、稳定性和聚合态。AVBF可直接捕获有机小分子亚甲蓝（MB）,其捕获率为15.0±2.0MB/AVBF,认为介于AVBF亚基单体之间的血红素参与捕获MB。较高浓度（40%;0%）的乙腈和丙酮均能使AVBF和鲨鱼肝铁蛋白（Liver ferritin of shark,SLF）释放不稳定亚基,但在较低浓度（20%;0%）的乙腈条件下,却需要借助来源于质谱仪的激光才能使AVBF或SLF释放不稳定亚基,并供质谱分析。AVBF亚基之间的相互作用强度明显低于SLF。铁蛋白亚基之间的相互作用强度高低与铁蛋白执行释放和储存铁的速率有关。
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