SPAP2,a transmembrane protein,is an Ig family receptor containing both ITIMs(immunoreceptor tyrosine-based inhibition motifs) and ITAMs(immunoreceptor tyrosine-based activation motifs).The extracellular portion of SPA...
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SPAP2,a transmembrane protein,is an Ig family receptor containing both ITIMs(immunoreceptor tyrosine-based inhibition motifs) and ITAMs(immunoreceptor tyrosine-based activation motifs).The extracellular portion of SPAP2 contains six immunoglobulin-like domains and its intracellular segment has two ITAMs and two *** alignment with the genomic database reveals that the SPAP2 gene contains 16 exons and is localized at chromosome ***2 is consisted of 734 amino acids,and the intercellular portion of SPAP2 contains 137 amino acids. Tyrosine-phosphorylated SPAP2 is specifically associated with Sh2 domain-containing tyrosine kinases and Sh2 domain-containing tyrosine phosphatases,which lead to the initiation of signal ***2CT gene was amplified by PCR with SPAP2 full-length DNA as the template and cloned to the pBluescript Ⅱ KS ***-2T-SPAP2CT,the expression vector of dissoluble fusion protein,was constructed and transferred into *** of *** fusion protein GST-SPAP2CT was expressed efficiently and purified by FFQ ion exchange chromatography and GSh affinity *** result indicates that we have constructed steady expression vector pGex-2T-SPAP2CT, which was expressed in *** molecular weight of the dissoluble fusion protein is 46 000,the productivity of GST-SPAP2CT protein is 10% and the purity is over 90% after the purification.
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