A full-length cDNA plasmid of foot-and-mouth disease virus (FMDV), designated pMCES, has been constructed by stably inserting genome-length cDNA into the low-copy-number plasmid vector pMCIS under the control of a T7 ...
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A full-length cDNA plasmid of foot-and-mouth disease virus (FMDV), designated pMCES, has been constructed by stably inserting genome-length cDNA into the low-copy-number plasmid vector pMCIS under the control of a T7 RNA polymerase promoter. The genome of the ES strain was used to synthesis the template. In order to identify the recombinant virus, a nucleotide mutation leading to a unique Smal site at position 477 was introduced into the clone at the 5' non-coding region. T7 RNA polymerase transcripts of the ligated full-length cDNA template in vitro were infectious when transfected into BHK-21 cells. Recovered virus was identified by RT-PCR, restriction enzyme analysis. It was displayed the similar cytopathic effect and plaque to the original virus. Furthermore, the virulence of the recovered virus was identified in the sucking mice and it was found the same as the original virus.
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