A pair of primers containing BamHI and XhoI sites was designed to amplify n gene of 1149bp from the positive pMD18-TN plasmid of transmissible gastroenteritis virus n gene by *** product was subcloned into multiple cl...
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A pair of primers containing BamHI and XhoI sites was designed to amplify n gene of 1149bp from the positive pMD18-TN plasmid of transmissible gastroenteritis virus n gene by *** product was subcloned into multiple cloning sites of pET-30a containing 6 His·*** plasmid pET-30a of n gene named pET30a-N was transfected into *** BL21 and the bacteria was induced with IPTG at 37℃.It was demonstrated by SDS-PAGE that recombinant N protein expressed in *** BL21 was soluble protein and its molecular weight was about *** result of Western blot test showed that immunoactivity of the recombinant N protein was the same to that of the natural N protein.
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