Endothelium lines vascular walls and regulates vascular *** endothelial cells sense and respond to chemical and mechanical stimuli in the circulation,and couple the stimulus signals to vascular smooth muscles,in which...
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Endothelium lines vascular walls and regulates vascular *** endothelial cells sense and respond to chemical and mechanical stimuli in the circulation,and couple the stimulus signals to vascular smooth muscles,in which several membrane receptors and ion channels play a *** about these surface-expressing proteins may help to understand endothelium function and its role in certain vascular *** we show a K+current in primarily cultured pulmonary arterial endothelial cells(PAECs)that is regulated by the Ca2+/calmodulin(Ca M)-dependent protein kinase Ⅱ(Ca MKⅡ).In whole-cell voltage clamp,the PAECs showed large inward rectifier K+currents that were sensitive to micromolar concentrations of extracellular Ba2+,but insensitive to intracellular ATP and p *** excised inside-out patches,an inward rectifier K+current was observed with single-channel conductance 32.43±0.45 p S and Popen 0.27±*** PCR analysis showed strong expression of Kir2.1 in the endothelial *** current did not respond to intracellular GDP-β-S and extracellular cholera *** vascular regulators were *** K+current was insensitive to carbachol,neither to the e NOS inhibitor *** to the Ca M inhibitor W-7 and Ca MKⅡ inhibitor KN-62 strongly suppressed the ***,vasodilation was suppressed by W-7 and Ba2+in isolated and perfused pulmonary arterial ***,these results suggest that the PAECs express an inward rectifier K+current that is likely to be Kir2.1,and such a K+channel appears to be targeted by Ca MKⅡ-dependent intracellular signaling systems.
目的:研究甲基化CpG结合结构蛋白2(methyl-CpG-binding domain protein 2,MBD2)在胃癌的表达及靶向抑制。方法:免疫组化法对40例胃癌及配对胃黏膜组织,免疫荧光法对胃癌细胞SGC-7901行MBD2表达研究。构建3个靶向抑制MBD2的siRNA(MBD2-si...
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目的:研究甲基化CpG结合结构蛋白2(methyl-CpG-binding domain protein 2,MBD2)在胃癌的表达及靶向抑制。方法:免疫组化法对40例胃癌及配对胃黏膜组织,免疫荧光法对胃癌细胞SGC-7901行MBD2表达研究。构建3个靶向抑制MBD2的siRNA(MBD2-siRNA-1、MBD2-siRNA-2、MBD2-siRNA-3)。转染MBD2-siRNA入SGC-7901,RT-PCR及Western blot验证MBD2的抑制效果。噻唑蓝比色法检测SGC-7901增殖率。结果:胃癌组织MBD2表达较对照胃黏膜组织增强(χ2=6.646,P=0.010);且低分化胃癌与伴幽门螺杆菌感染的胃癌中MBD2表达更强(χ2值分别为9.730、8.700,P值分别为0.01和0.04)。MBD2蛋白在胃癌胞核分布。MBD2-siRNA可成功转染SGC-7901,并成功筛选靶向抑制MBD2的siRNA(MBD2-siRNA-2)。发现转染MBD2-siRNA-2后96 h,SGC-7901增值率被抑制。结论:MBD2在胃癌表达增强,抑制MBD2可能抑制胃癌增殖。
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