Endothelium lines vascular walls and regulates vascular *** endothelial cells sense and respond to chemical and mechanical stimuli in the circulation,and couple the stimulus signals to vascular smooth muscles,in which...
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Endothelium lines vascular walls and regulates vascular *** endothelial cells sense and respond to chemical and mechanical stimuli in the circulation,and couple the stimulus signals to vascular smooth muscles,in which several membrane receptors and ion channels play a *** about these surface-expressing proteins may help to understand endothelium function and its role in certain vascular *** we show a K+current in primarily cultured pulmonary arterial endothelial cells(PAECs)that is regulated by the Ca2+/calmodulin(Ca M)-dependent protein kinase Ⅱ(Ca MKⅡ).In whole-cell voltage clamp,the PAECs showed large inward rectifier K+currents that were sensitive to micromolar concentrations of extracellular Ba2+,but insensitive to intracellular ATP and p *** excised inside-out patches,an inward rectifier K+current was observed with single-channel conductance 32.43±0.45 p S and Popen 0.27±*** PCR analysis showed strong expression of Kir2.1 in the endothelial *** current did not respond to intracellular GDP-β-S and extracellular cholera *** vascular regulators were *** K+current was insensitive to carbachol,neither to the e NOS inhibitor *** to the Ca M inhibitor W-7 and Ca MKⅡ inhibitor KN-62 strongly suppressed the ***,vasodilation was suppressed by W-7 and Ba2+in isolated and perfused pulmonary arterial ***,these results suggest that the PAECs express an inward rectifier K+current that is likely to be Kir2.1,and such a K+channel appears to be targeted by Ca MKⅡ-dependent intracellular signaling systems.
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