Baicalin is a major bioactive ingredient from the roots of Scutellaria baicalensis Georgi and has various pharmacological actions such as anti-apoptosis and anti-oxidative ***,an involvement of baicalin in the ethanol...
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Baicalin is a major bioactive ingredient from the roots of Scutellaria baicalensis Georgi and has various pharmacological actions such as anti-apoptosis and anti-oxidative ***,an involvement of baicalin in the ethanol-induced cerebral impairment remains ***,here we explored that the neuroprotective effects of baicalin on ethanol-induced impairment in C57BL/6 mice model with Lieber-De Carli liquid diet including *** utilizing Morris water maze test,we found that baicalin attenuated the cognitive dysfunction of mice treated with *** discuss the anti-apoptosis effects of baicalin on ethanol mediated neuronal impairment,histopathological examination such as Nissl staining and Neu N immunohistochemistry,molecular biological analysis such as Western Blot and quantitative PCR were used to evaluate the apoptosis of hippocampal *** analysis of above results showed that baicalin could attenuate apoptosis of hippocampal neurons after ethanol *** explore the anti-oxidative mechanism of baicalin in hippocampal neuronal impairment caused by ethanol,the oxidative stress injury of hippocampal neurons was evaluated by measuring levels of malondialdehyde(MDA),activities of catalase(CAT),Cu/Zn-superoxide dismutase(SOD),Mn-SOD,glutathione(GSH)and glutathione peroxidase(GSH-PX).In addition,Western blot analysis showed that baicalin modulated the protein expressions of mitogen-activated protein kinases(MAPKs),including ERK,JNK and ***,these results suggest that baicalin alleviates ethanol-mediated hippocampal neuronal impairment by anti-apoptosis and anti-oxidative,and then improves cognitive function of mice treated byethanol,which is likely to be related to MAPK signal pathway.
Endothelium lines vascular walls and regulates vascular *** endothelial cells sense and respond to chemical and mechanical stimuli in the circulation,and couple the stimulus signals to vascular smooth muscles,in which...
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Endothelium lines vascular walls and regulates vascular *** endothelial cells sense and respond to chemical and mechanical stimuli in the circulation,and couple the stimulus signals to vascular smooth muscles,in which several membrane receptors and ion channels play a *** about these surface-expressing proteins may help to understand endothelium function and its role in certain vascular *** we show a K+current in primarily cultured pulmonary arterial endothelial cells(PAECs)that is regulated by the Ca2+/calmodulin(Ca M)-dependent protein kinase Ⅱ(Ca MKⅡ).In whole-cell voltage clamp,the PAECs showed large inward rectifier K+currents that were sensitive to micromolar concentrations of extracellular Ba2+,but insensitive to intracellular ATP and p *** excised inside-out patches,an inward rectifier K+current was observed with single-channel conductance 32.43±0.45 p S and Popen 0.27±*** PCR analysis showed strong expression of Kir2.1 in the endothelial *** current did not respond to intracellular GDP-β-S and extracellular cholera *** vascular regulators were *** K+current was insensitive to carbachol,neither to the e NOS inhibitor *** to the Ca M inhibitor W-7 and Ca MKⅡ inhibitor KN-62 strongly suppressed the ***,vasodilation was suppressed by W-7 and Ba2+in isolated and perfused pulmonary arterial ***,these results suggest that the PAECs express an inward rectifier K+current that is likely to be Kir2.1,and such a K+channel appears to be targeted by Ca MKⅡ-dependent intracellular signaling systems.
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