Crotalaria witches’-broom phytoplasma (CrWB) collected from Hainan Province,was classified and identified by sequencing techniques and Virtual *** different type universal primer pairs of phytoplasma were used to amp...
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Crotalaria witches’-broom phytoplasma (CrWB) collected from Hainan Province,was classified and identified by sequencing techniques and Virtual *** different type universal primer pairs of phytoplasma were used to amplify a 1.8 kb fragment of 16S rDNA and a 1.3 kb fragment of rp *** PCR-amplified products were cloned and *** results of sequencing and homologuous comparison with other phytoplasmas showed that Crotalaria witches’-broom phytoplasma shared 99.9 % similarity with peanut witches’-broom phytoplasma in 16S rRNA gene and 99.9% in rp *** witches’-broom phytoplasma is sorted into Peanut witches’-broom group(16SrII),16SrII-A,according to the results of Virtual RFLP of 16S rDNA,phylogenetic analysis of 16S rRNA and rp gene,which is related to *** australasiae.
【目的】探究光周期和温度对美国白蛾滞育的影响,明确诱导美国白蛾滞育的主控因子和幼虫敏感龄期,为深入研究光周期诱导美国白蛾滞育的分子机制和开发新型防治技术奠定基础。【方法】设置4个温度(21、23、25和27℃)和7个光周期(L8∶D16、L10∶D14、L12∶D12、L13∶D11、L14∶D10、L15∶D9和L16∶D8),统计和比较28种不同处理条件下美国白蛾的滞育结果,阐明光周期和温度对美国白蛾滞育和临界光周期的影响;23℃条件下,在美国白蛾幼虫不同发育阶段分别给予长光照(L16∶D8)和短光照(L10∶D14)交替饲养处理,统计和比较32种不同处理下美国白蛾的滞育结果,明确光周期诱导美国白蛾滞育的敏感龄期。【结果】在21、23、25和27℃条件下,光照时间小于13 h,可导致美国白蛾滞育发生,光照时间达到16 h,美国白蛾个体继续发育而无滞育发生,光照时间在13~16 h之间时,滞育同时受光周期和温度调控,且滞育率随光周期增长或温度升高均呈下降趋势。21、23、25和27℃条件下的临界光周期分别为14 h 58 min∶9 h 02 min(L∶D)、14 h 45 min∶9 h 15 min(L∶D)、14 h 35 min∶9 h 25 min(L∶D)和14 h 24 min∶9 h 36 min(L∶D),临界光周期随温度升高逐渐缩短。短光照对美国白蛾滞育诱导具有累积效应,美国白蛾幼虫3龄、4龄和5龄对光周期诱导滞育最为敏感。【结论】美国白蛾属典型的长日照发育-短日照滞育型昆虫,光周期在美国白蛾滞育诱导中发挥主导作用,温度伴随光周期发挥作用,低温和短光照利于滞育发生;临界光周期受温度调控,随温度升高逐渐缩短;美国白蛾对光周期诱导滞育具有累积效应,感受光周期最敏感的阶段为幼虫3~5龄。
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