To develop a HPLC-DAD-ESI-TOF/MS analysis method for the determination of gentiopicroside and loganic acid in Radix gentianae samples and for the research of their *** samples were extracted using ASE for 10 min under...
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To develop a HPLC-DAD-ESI-TOF/MS analysis method for the determination of gentiopicroside and loganic acid in Radix gentianae samples and for the research of their *** samples were extracted using ASE for 10 min under 100 ℃ and 9.65 MPa,and divided into water phase and chloroform phase and analyzed them with HPLC-DAD-ESI-TOF/MS method *** on this method,the HPLC fingerprints of Radix gentianae were *** the spectrogram and mass spectrum of the chromatogram peak with the reference value,three compounds in water phase were identified as gentiopicroside,asafetida acid and loganic *** is no report of the compounds in chloroform *** content of gentiopicroside and loganic acid in samples of different groups were determined,*** fingerprints were compared by the software of the similarity evaluation system for chromatographic *** water phase fingerprint congruence coefficients of samples from six different areas were above 0.90,however,the chloroform phase fingerprint congruence coefficients were within *** method can be used for determination of potent component in Radix gentianae and its quality *** gentianae from different producing areas have the largest diversities,and the diversities embodied in the content of chloroform phase compounds.
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