超高效液相色谱-三重四级杆质谱联用技术(ultra-highperformanceliquid chromatography-tandem mass spectrometry,UPLC-MS/MS),具有灵敏度高、精准性好、可微量检测的优势。利用该技术优势,研究建立了灵芝三萜的系列靶向检测方法。该...
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超高效液相色谱-三重四级杆质谱联用技术(ultra-highperformanceliquid chromatography-tandem mass spectrometry,UPLC-MS/MS),具有灵敏度高、精准性好、可微量检测的优势。利用该技术优势,研究建立了灵芝三萜的系列靶向检测方法。该方法选取了灵芝中含量较高、活性较好的灵芝酸、烯酸、醇、醛、酯等共计43种三萜作为标准对照品,采用两种反相色谱柱,在两种电离模式下分别建立了动态多反映监测(DynamicMultipleReactionMonitoring,DMRM)模式分析方法。经方法学验证,两种方法的日间与日内精密度、样品重复性、稳定性及加样回收率均符合要求。方法建成后,对国内主要灵芝栽培种赤芝、紫芝、松杉灵芝、白肉灵芝和无柄灵芝进行了三萜种类和含量的分析。结果表明,作为主栽品种和国家法规种,代料栽培的赤芝中总三萜含量普遍高于椴木栽培的赤芝,但椴木栽培的灵芝的中性三萜含量相对高于代料栽培。对赤芝不同生长时期采集的子实体的三萜含量检测结果均发现,随着子实体生长至成熟,酸性三萜含量有降低的趋势,而中性三萜含量逐渐增加。松杉灵芝、白肉灵芝和无柄灵芝中主要含有中性三萜化合物,但含量明显低于赤芝。人工栽培的灵芝中,紫芝子实体中含有最少种类和最低含量的被检测三萜化合物。此外,研究还对采集自各地的30个批次的赤芝孢子粉中的三萜进行了定性定量分析,发现孢子粉中三萜种类丰富,但含量普遍较低,在0-267μg/g之间。
Hypsizygus marmoreus(Peck) H.E. Bigelow is an important industrialized mushroom in Asia,yet the lack of basic research on this fungus has hindered further development of its economic value. Research on gaseous hydroge...
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Hypsizygus marmoreus(Peck) H.E. Bigelow is an important industrialized mushroom in Asia,yet the lack of basic research on this fungus has hindered further development of its economic value. Research on gaseous hydrogen has progressed from focusing on its role as a fuel to its role as matter that is able to regulate biological and pharmacological functions. For instance, hydrogen-rich water(HRW) inhibited glucose-and α, β-dicarbonyl compound-induced ROS production in rat kidney homogenates in vitro(Katakura et al. 2012). HRW also significantly decreased the ROS content, maintained the biomass and polar growth morphology of mycelium, and decreased secondary metabolism in G. lucidum under HAc-induced oxidative stress(Ren et al. 2016). However,there is no research to date on the effects of HRW on H. marmoreus mycelial growth and fruiting body *** the vegetative stage, HRW was firstly employed to characterize physiological roles and molecular mechanisms of H2 in the alleviation of three different stresses in basidiomycete H. marmoreus. Our results showed that HRW treatment, of which the H2 concentration was 0.8 m M, significantly reduced the toxicities of CdCl2, Na Cl and H2 O2, leading to significantly improved mycelial growth and biomass. These beneficial effects could be attributed to a significantly decreased formation of malondialdehyde(MDA). Besides, HRW treatment significantly increased the activities of antioxidants(SOD, CAT and GR) as well as the gene expressions of these antioxidants(SOD, CAT, and GR) at the m RNA level. At the reproductive stage, mycelia injured by scratching were treated with HRW to investigate the involvement of the redox system in fruiting body *** to the control group, damaged mycelia treated with HRW regenerated earlier and showed significantly enhanced fruiting body production. Antioxidant capacity increased significantly in damaged mycelia after HRW treatment, as indicated by higher antioxidant enzyme activit
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