叶片结构性状是植物适应环境和维持生命活动的基础。为探究荒漠建群种灰杨(Populus pruinosa Schrenk)叶片结构性状的空间变异及其与环境因子的关系,以新疆11个天然灰杨居群为研究对象,对灰杨叶片的形态、解剖性状进行研究,用Pearson相关性分析、植物性状网络(PTN)、冗余分析(RDA)等方法对灰杨叶片结构性状的空间变异与气候、土壤因子的关系进行分析。结果表明:1) 灰杨叶片的解剖性状比形态性状的变异性更高,出叶强度(LIM)和主脉维管束面积(MVBA)具有较高的变异系数。2) 形态性状和解剖性状间存在显著的协同与权衡关系,在性状网络中,MVBA为中心性状,可能起着影响灰杨叶片结构的中心调控作用。3) 年均温、年降水量和土壤全钾含量对灰杨叶片结构性状的影响较大,在较干旱生境下灰杨表现出小比叶面积和大叶组织密度的资源保守型策略,相对湿润时则采取高比叶面积和低叶组织密度的资源获取型策略。Leaf structural traits are fundamental to plant adaptation and the maintenance of life processes. To investigate the spatial variation in leaf structural traits of the desert tree species Populus pruinosa Schrenk and its association with environmental factors, 11 natural P. pruinosa populations in Xinjiang were selected as the research objects to study the morphology and anatomical traits of P. pruinosa leaves. Pearson correlation analysis, plant trait network, redundancy analysis and other methods were performed to analyze the spatial variation in leaf traits and their relationship with climatic and soil factors. The results showed that: 1) The leaf anatomical traits of P. pruinosa exhibited greater variability than their morphological traits, and leafing intensity and main vein vascular bundle area showed particularly high coefficients of variation. 2) A significant synergistic and trade-off relationship was identified between morphological and anatomical traits, with main vein vascular bundle area emerged as a central trait within the structural trait network. This suggested a pivotal regulatory role in shaping the leaf structure of P. pruinosa. 3) The annual average temperature, annual precipitation, and soil total potassium content significantly influenced the leaf structural traits of P. pruinosa. In drier habitats, P. pruinosa exhibited a resource conservation strategy of small SLA and large LTD, whereas in relatively humid environments, an opposite resource acquisition strategy was adopted.
基因组编辑技术(Genomeeditingtechnology)是一种通过人工手段在基因组水平对DNA序列进行改造的遗传操作技术,包括特定DNA片段的插入、敲除、替换和点突变。其中,依赖核酸酶的基因组编辑技术的基本原理是在基因组的特定位置产生双链DNA断裂(Double-strandedbreak,DSB)后通过非同源末端连接(Non-homologous end joining,NHEJ)或同源重组(Homologous recombination,HR)的方式进行修复。随着对核酸酶更深入的研究,基因组编辑技术也得到了快速发展,其中最常使用的核酸酶主要包括巨型核酸酶、锌指核酸酶、转录激活因子样效应物核酸酶以及成簇的规律间隔的短回文重复序列相关蛋白(Clusteredregularly interspaced short palindromic repeats(CRISPR)-associated proteins(Cas)。文中在介绍上述基因组编辑技术的发展及作用原理的基础上,主要综述了CRISPR/Cas9系统在基因功能鉴定、疾病模型建立、基因治疗和免疫治疗等应用领域的研究进展,并对其未来发展进行了展望。
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