目的研究2型糖尿病大鼠肠系膜微血管和微血流的变化。方法Otsuka-long-evans-tokushi ma fatty(OLETF)2型糖尿病大鼠33只,对照组long-evans tokushi ma otsuka(LETO)非糖尿病大鼠7只,全麻状态下,使用带电脑图像处理装置的微循环活体观...
详细信息
目的研究2型糖尿病大鼠肠系膜微血管和微血流的变化。方法Otsuka-long-evans-tokushi ma fatty(OLETF)2型糖尿病大鼠33只,对照组long-evans tokushi ma otsuka(LETO)非糖尿病大鼠7只,全麻状态下,使用带电脑图像处理装置的微循环活体观察电视显微镜对糖尿病和非糖尿病对照组大鼠作活体肠系膜微循环观察。检测微血管分支数目、微动脉及微静脉口径、微静脉中沿壁滚动与贴壁黏附的白细胞数、微动脉边流和轴流的宽度、内皮细胞厚度等。实验结果经电视录像记录后用图像处理系统定量测定。结果糖尿病大鼠肠系膜微血管分支数与对照组大鼠比较减少24.5%(P<0.01),微血管分支数与血糖水平呈负相关(r=-0.44,P<0.05);糖尿病大鼠微动脉边流宽度小于对照组,边流与管径的比值显著减少(P<0.01),沿壁滚动与贴壁黏附白细胞明显增加(P<0.01),免疫细胞化学结果显示糖尿病大鼠肠系膜微血管内皮细胞表面血管细胞黏附分子1(Vascular cell adhesion molecule-1,VCAM-1)表达明显增强。结论糖尿病大鼠肠系膜微循环出现明显的微血管与微血流形态、结构和功能障碍,这些变化是糖尿病微血管病和各种并发征发生的基础。
In present review,the history of microangiopathy discovery and it’s modern concept were introduced and the pathophysiological changes of some vital organs in diabetes were *** roles of oxidation stress,distur-bances ...
详细信息
In present review,the history of microangiopathy discovery and it’s modern concept were introduced and the pathophysiological changes of some vital organs in diabetes were *** roles of oxidation stress,distur-bances of microvascular functions,microvascular endothelial cell damage,PKC and PPARγ in pathogenesis of diabetic microangiopathy were also discussed.
Reactive oxygen species(ROS)are crucial in the pathogenesis of diabetic nephropathy(DN).X-box binding protein 1(XBP1),a key mediator of endoplasmic reticulum(ER)stress,has been proved having the capability of preventi...
详细信息
Reactive oxygen species(ROS)are crucial in the pathogenesis of diabetic nephropathy(DN).X-box binding protein 1(XBP1),a key mediator of endoplasmic reticulum(ER)stress,has been proved having the capability of preventing oxidative *** this study,the effects of spliced XBP1(XBP1S)on high glucose(HG,30 mmol/L D-glucose)induced ROS production and apoptosis were investigated in cultured renal mesangial cells(MCs).ROS level was detected by dihydroethidium(DHE)fluorescent probe *** blot were used to evaluate the XBP1S,pro-caspase3,cleaved-caspase3,Bax and Bcl2 protein *** cytometry was used to detect cell apoptosis and *** of recombinant adenovirus vector carrying XBP1S gene(Ad-XBP1S)was used to upregulate XBP1S *** results showed that HG treatment significantly reduced XBP1S protein levels in the cultured *** Cytometry results showed that the Annexin-V positive cell was increased significantly after HG *** of Ad-XBP1S reversed HG-induced ROS ***-XBP1S trasnfection suppressed the HG-induced increases in both total caspase 3 and cleaved caspase 3;Similarly,the Ad-XBP1S trasnfection reversed the HG-induced increase in the ratio of Bax/*** blot results demonstrated that Diphenylene-chloride iodonium(DPI,10-6 mol/L),an inhibitor of NADPH oxidase,inhibited HG induced Bax/Bcl2,pro-caspase3 and cleaved-caspase3 protein *** results suggested that suppression in XBP1S pathway of ER stress was involved in HG-induced *** might be a downstream mediator of XBP1S in regulating cell apoptosis.
暂无评论