Interleukin1β (IL 1β) is a proinflammatory cytokine and plays an important role in the pathogenesis of cerebral ischemia. The expression of IL 1β and its receptor antagonist (IL 1Ra) after cerebral ischemia is not ...
详细信息
Interleukin1β (IL 1β) is a proinflammatory cytokine and plays an important role in the pathogenesis of cerebral ischemia. The expression of IL 1β and its receptor antagonist (IL 1Ra) after cerebral ischemia is not well defined so far. The aim of present study was to explore the effect of electroacupuncture (EA) on the expression of IL 1β and IL 1Ra in rats after middle cerebral artery occlusion (MCAo) and reperfusion. Using in situ hybridization and RT PCR techniques, it was found that in the MCAo group the expression of IL 1β mRNA was markedly increased at 2 hr, 6 hr and 12 hr after reperfusion in the ischemic cerebral cortex compared with normal group. The IL 1Ra mRNA expression was rapidly induced by MCAo, and also increased significantly at 12 hr, reaching a peak level at 24 hr of reperfusion in ischemic cortex. In ischemic striatum the IL 1Ra mRNA was increased only at 12 hr after ischemia/reperfusion and decreased significantly at 24 hr after ischemia/reperfusion. In EA + MCAo group the expression of IL 1β mRNA in ischemic cortex was significantly decreased at 2 hr, 6 hr and 12 hr; but the expression of IL 1Ra mRNA was increased significantly compared with MCAo group 24 hr after reperfusion in the cerebral cortex and stratium. Our results indicated that EA stimulation of "Shuigou" (GV 26) and "Baihui" (GV 20) acupoints could downregulate the IL 1β mRNA expression and upregulate the IL 1Ra mRNA expression in cerebral ischemic rats, which might be the neuroprotective effect of EA on cerebral ischemia, and one of the mechanisms of EA anti-ischemia.
Our previous work demonstrated that microinjection of OFQ into PAG antagonized e lectroacupuncture (EA) analgesia; increased the release of Glu in spinal dorsal horn and decreased the release of GABA, 5 HT and NE; and...
详细信息
Our previous work demonstrated that microinjection of OFQ into PAG antagonized e lectroacupuncture (EA) analgesia; increased the release of Glu in spinal dorsal horn and decreased the release of GABA, 5 HT and NE; and significantly facilita t ed c fibre response and post discharge of WDR neurons of spinal dorsal horn in t he rats. The aim of current study is to observe the effect of microinjection of OFQ into PAG and simultaneous EA on the release of monoamines and Amino acids in spinal dorsal horn in the rats, by the methods of nucleus injection, micro dia lysis and HPLC EC. The results were as follows: After microinjection of OFQ into PAG and simultaneous EA, extracellular concentr ation of Glu was significantly decreased, whereas extracellular concentratio ns of GABA, 5 HT and 5 HIAA, NE and MHPG were significantly increas ed (P<0.05～0.01), indicating significant differences in comparison wi t h OFQ control group (microinjection of OFQ into PAG alone) in the corresponding phases (P<0.5～0.001). The results suggest there are opposite effects on the descending pain modulating system between OFQ in the brain and EA, so this may be one of the mechanisms of OFQ in the brain antagonizing EA analgesia.
暂无评论