Objective Munc18 is considered as an intracellular protein that plays an important role in exocytosis of *** studies have demonstrated the presence of autoantibodies against Munc18 in a subgroup of Rasmussen’s enceph...
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Objective Munc18 is considered as an intracellular protein that plays an important role in exocytosis of *** studies have demonstrated the presence of autoantibodies against Munc18 in a subgroup of Rasmussen’s encephalitis ***,the machinery of Munc18 autoimmunity is still *** present study was aimed to investigate Munc18 release from primary cultured neurons,Munc18 distribution on the outer plasma membrane of neurons,and the neurotoxicity of Munc18 *** The cerebral cortical neurons from embryonic day 17 SpragueDawley rats were prepared and cultured in neurobasal *** proteins in culture medium were precipitated with 10% trichloroacetic acid,and analyzed by *** proteins on neuronal surface were biotinylated with EZ-Link-sulfoNHS-LC-Biotin,and collected with avidin-conjugated agarose beads followed by immunoblotting *** cell surface immunofluorescent staining,the living neurons were labeled with anti-Munc18 antibody at 4 °*** injury was assessed by lactate dehydrogenase（LDH） *** Munc18 was detected in culture medium by immunoblotting *** treatment with 50 μmol/L glutamate for 1 h,Munc18 content in medium was ***,β-actin and syntaxin1 were not detected in culture medium,and LDH release was not significantly ***,glutamate enhanced Munc18 distribution on outer plasma *** neuron staining also demonstrated the localization of Munc18 on neuronal surface after glutamate treatment,especially at contacting regions between ***-induced increase of surface Munc18 distribution was suppressed by NMDA receptor antagonist MK801,but not by AMPA receptor antagonist ***,compared with c-Fos antibody,Munc18 antibody could induce neuronal injury,when culture medium contained the components of *** A portion of Munc18 can be released from neurons or distributed on neuronal surface,which can be enha
Patients with medulloblastomas after primary therapy have a particularly poor prognosis, with a median survival of less than 6 months;the 2-year survival rate of approximately 9%. Th17 cells (a new member of the CD4+ ...
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Patients with medulloblastomas after primary therapy have a particularly poor prognosis, with a median survival of less than 6 months;the 2-year survival rate of approximately 9%. Th17 cells (a new member of the CD4+ effector T-cell family) have substantially advanced our understanding of T cell-mediated immunity. The aim of this study was to investigate the role of T (Treg) and Th17 cells in medulloblastom immune pathology and therapy.
Objective To assess whether quick cognitive screening test （QCST） could quickly identify mild cognitive impairment （MCI）. Methods QCST and a full set of standardized neuropsychological tests, including mini-mental...
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Objective To assess whether quick cognitive screening test （QCST） could quickly identify mild cognitive impairment （MCI）. Methods QCST and a full set of standardized neuropsychological tests, including mini-mental state examination （MMSE） and montreal cognitive assessment （MoCA） were performed. A total number of 121 cases of MCI [41 cases of amnestic MCI-single domain （aMCI-s）; 44 of amnestic MCI-multiple domain （aMCI-m）; 36 of nonamnestic MCI （naMCI）], 79 cases of mild Alzheimer’s disease （AD） and 186 healthy elderly volunteers were employed in the present study. All the participants （55-85 years old） had an educational level no less than 5 years. QCST subtests included word list recall, naming test, animal fluency test, similarity test, color trail-1min, clock drawing test, finger construction test, and digit span test. The total score of QCST was 90 points, 10 points for each index of subtests. Results The total scores of QCST in MCI, AD and the control groups were （58.13±8.18）, （44.53±10.54） and （72.92±6.85） points, respectively. According to the educational level, the cut off scores of participants with an educational level of 5-8 years, 9-12 years and more than 13 years were 63, 65 and 68 points, respectively. The sensitivity and specificity of QCST in detection of MCI were 87.6% （85.7% for aMCI-s, 90.1% for aMCI-m and 89.5% for naMCI） and 84.3%, respectively. The area under the curve was 0.923 （95% CI： 0.892-0.953）. Delayed memory, color trail-1min and similarity test could help distinguish between aMCI and naMCI. Conclusion QCST may have a good sensitivity and specificity for MCI detection, which warrants its further clinical application.
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