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第八届全国神经外科年会

作者：朱剑虹汤奇胜周良辅潘力高亮吴惺吴学海汤海亮陈露萍沈亦雯复旦大学附属华山医院神经外科上海市神经外科临床医学中心上海市神经外科急救中心医学神经生物学国家重点实验室复旦大学脑科学研究院上海医学院200040

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中枢神经系统血管母细胞瘤的干细胞起源和发病学关键步骤(英文)

中枢神经系统血管母细胞瘤的干细胞起源和发病学关键步骤(英文)

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第四届中国肿瘤学术大会暨第五届海峡两岸肿瘤学术会议

作者：朱剑虹谢嵘陈露萍沈亦雯毛颖周良辅复旦大学附属华山医院神经外科复旦大学上海医学院医学神经生物学国家重点实验室上海市神经外科临床医学中心复旦大学附属华山医院神经外科复旦大学上海医学院医学神经生物学国家重点实验室上海市神经外科临床医学中心复旦大学附属华山医院神经外科复旦大学上海医学院医学神经生物学国家重点实验室上海市神经外科临床医学中心复旦大学附属华山医院神经外科复旦大学上海医学院医学神经生物学国家重点实验室上海市神经外科临床医学中心复旦大学附属华山医院神经外科复旦大学上海医学院医学神经生物学国家重点实验室上海市神经外科临床医学中心复旦大学附属华山医院神经外科复旦大学上海医学院医学神经生物学国家重点实验室上海市神经外科临床医学中心

＜正＞Objective: To better understand the cell origin and pathogenetic step of central nervous system he-mangioblastomas （CNS HB）. Methods: 14 VHL-associ-ated CNS HB, 21 none VHL-associated CNS HB and 15 normal brain tissues were collected and all the specimens were sectioned and subjected to routine histology and im-munohistochemistry analysis. Avidin-biotin-complex immunoperoxidase was used to evaluate the expression of CD31, CD34, CD117, CD133, Nestin, erythropoietin （EPO） in CNS HB. Another three mixed samples collected from 5 VHL-associated CNS HB, 7 none VHL-asociated CNS HB and 7 normal brain tissues were analyzed by using Oligo cDNA microarray to screen the stem cells markers, of which the gene expression levels had distinct differences among VHL associated HB, none VHL associated HB and normal brain tissues. RT-PCR and Western blot was used to confirm the validity of the results. Results: After inunu-nochemistry analysis, all CNS HB pathological sections were observed to express stem cells markers including CD31, CD34, CD117, CD133, Nestin. The results of cDNA microarray showed higher gene expression levels of several important stem cells markers including ABCG2, AXIN1, BMP2, CD3, CD4, etc of CNS HB than those of normal brain tissues and no expression difference of the markers was found between VHL and none VHL-associated HB. Both immunochemistry and cDNA microarray suggested that EPO was highly expressed in CNS HB but the expression level of VHL-associated HB was higher than that of none VHL-associated HB. Conclusions: The study suggests that CNS HB can express markers of mes- enchymal stem cells and may have an origin of mesenchy-mal stem cells. The expression of EPO may be a critical pathogenetic step as a cell proliferation stimulus but has different contributions in VHL and none VHL-associated HB.

关键词：血管母细胞瘤干细胞发病学 VHL EPO

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中枢神经系统血管母细胞瘤蛋白质组学研究(英文)

中枢神经系统血管母细胞瘤蛋白质组学研究(英文)

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第四届中国肿瘤学术大会暨第五届海峡两岸肿瘤学术会议

作者：谢嵘朱剑虹复旦大学附属华山医院神经外科复旦大学上海医学院医学神经生物学国家重点实验室上海市神经外科临床医学中心复旦大学附属华山医院神经外科复旦大学上海医学院医学神经生物学国家重点实验室上海市神经外科临床医学中心

Obejctive: To establish detailed protein expression maps of central nervous system bemangioblastomas (CNS HB) and identify discrepant proteins between HB and normal brain tissues by using proteomics analysis so as to better understand the histological origin of CNS ***: Ten individual brain regions with five HB tissues (HB group) and five normal cerebellar tissues (control group) were *** proteins were extracted from the samples and process of two dimensional gel *** excision,in- gel digestion, MALDI - TOF MS and bioinformatics analysis were carried out. Results: After 2 - DE, high - resolution maps containing about 600 protein spots of HB and normal brain tissues were *** 2 - DE maps show a satisfactory homology between HB and normal brain tissues. By using the ImageMaster 2D software, 115 discrepant protein spots between HB and normal brain tissues could be selected and 87 of them were successfully identified by MALDI - TOF MS. Among them,46 proteins expressions were up - regulated while 41 were down - regulated. According to their functional role,the identified proteins could be divided into several groups including transport,protein metabolism and folding,glycolysis and stem cell proteins,et *** results of immunohistochemistry staining of EPO, Vimentin and 14-3-3 proteins could authenticate the validity of the proteomics analysis in this study. Conclusions: The process of CNS HB occurring is an intricate, multicomponent, multifactorial and multi-step process which is mediated by a variety of *** HB as a kind of tumors may come from normal brain mesenchymal *** proteins such as EPO、Vimentin and 14-3-3 may play an important role in the occurring of HB.

关键词： CNS hemangioblastomas,stem cells,pathogenetics,VHL,EPO

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神经干细胞和人脑再生医学

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中华医学杂志 2005年第36期85卷 2527-2529页

作者：朱剑虹复旦大学附属华山医院神经外科医学神经生物学国家重点实验室上海市神经外科临床医学中心 200040

近年来研究发现,神经再生是成年哺乳动物大脑中的生理现象.由成年神经干细胞生成的神经元不论是体外培养还是在体内都具有相应的电生理功能,并能够在损伤后进行神经再生修复[1-3].而且基因修饰干细胞还可用于神经系统疾病的基因治疗,表... 详细信息

近年来研究发现,神经再生是成年哺乳动物大脑中的生理现象.由成年神经干细胞生成的神经元不论是体外培养还是在体内都具有相应的电生理功能,并能够在损伤后进行神经再生修复[1-3].而且基因修饰干细胞还可用于神经系统疾病的基因治疗,表达外源性的神经递质、神经营养因子及代谢性酶,为许多难治性神经系统疾病提供了新的治疗途径。

关键词：神经干细胞再生医学神经再生修复神经系统疾病人脑神经营养因子基因治疗电生理功能研究发现

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