目的观察慢病毒介导的RNAi沉默IL-1Ⅰ型受体对实验性大鼠膝骨关节炎(osteoarthritis,OA)中的丝裂素原激活的蛋白激酶(mitogen-activated protein kinase,MAPK)途径的影响,探讨IL-1和MAPK在OA中的作用。方法40只SD大鼠随机分为A～D4组,每...
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目的观察慢病毒介导的RNAi沉默IL-1Ⅰ型受体对实验性大鼠膝骨关节炎(osteoarthritis,OA)中的丝裂素原激活的蛋白激酶(mitogen-activated protein kinase,MAPK)途径的影响,探讨IL-1和MAPK在OA中的作用。方法40只SD大鼠随机分为A～D4组,每组10只。A～C组行单侧前交叉韧带和内侧半月板前1/3切除术,分别于术后第7和9天,A组关节腔中注射慢病毒介导IL-1Ⅰ型受体有效RNA干扰片段,B组注射慢病毒介导IL-1Ⅰ型受体无效RNA干扰片段,C组单纯注射生理盐水。D组为正常动物不做处理。术后4周处死动物,对关节软骨的状况进行评分。取膝关节软骨组织,用Western blot方法检测软骨组织中p38、ERK1/2和JNK1/2的蛋白质表达水平。结果大体观察显示:A组软骨退行性变显著轻于B、C组(P<0.05),但高于D组(P<0.05)。Western blot显示,A组p38蛋白质表达水平显著低于B、C组(P<0.05),与D组没有显著性差异(P>0.05)。A组ERK1/2和JNK1/2的表达显著低于B、C组(P<0.05),但要显著高于D组(P<0.05)。结论慢病毒介导RNA干扰沉默IL-1Ⅰ型受体能抑制MAPK的表达,特别是对其中的p38蛋白质抑制更为明显。
Objective: To determine the acceptance and effects of the 7 minute neurocognitive screening battery used in Chinese old people. Methods: Three groups of subjects,the Alzheimer disease(AD) group, the normal control gro...
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Objective: To determine the acceptance and effects of the 7 minute neurocognitive screening battery used in Chinese old people. Methods: Three groups of subjects,the Alzheimer disease(AD) group, the normal control group, and the non-dementia neuro- or psychiatric disease group,were examined by the MMSE,FOM,the WISC-BD,WAIS-DS,the ADL and the 7 minute battery. Results: Compared with normal control group and non-demential patients group,the AD group got significantly higher score in Orientation subscale and lower scores in other subscales of the 7 minute battery. The scores of MMSE? FOM? RVR? DS and BD were negatively correlated with the Orientation subtest and positively correlated with other subtests of the 7 minute battery.
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