检索结果-内蒙古大学图书馆

Chemistry of the Chinese herbal medicine Puerariae Radix(Ge-Gen): A review

Journal of Chinese Pharmaceutical Sciences 2014年第6期23卷 347-360页

作者：宋玮李艳姣乔雪钱薏叶敏北京大学医学部药学院天然药物及仿生药物国家重点实验室北京100191

Puerariae Radix （Ge-Gen in Chinese） is a popular traditional Chinese herbal medicine derived from the driedroots ofPueraria lobata （Willd.） Ohwi or P. thomsonii Benth. It shows significant pharmacological activities in the treatment ofcardiovascular diseases, hypertension, and diabetes. This review summarizes progress in the chemistry study of Puerariaspecies, mainly covering the classes of flavonoids, puerosides, coumarins, triterpenoids, and triterpene saponins. Qualitative andquantitative chemical analyses are also covered.

关键词： Pueraria lobata Pueraria thomsonii Puerariae Radix Chemical constituents Chemical analysis

来源：评论

学校读者我要写书评

暂无评论

Discovery of angucyclinone polyketides from marine actinomycetes with a genomic DNA-based PCR assay targeting type Ⅱ polyketide synthase

引用

Journal of Chinese Pharmaceutical Sciences 2017年第3期26卷 173-179页

作者：杨小燕金晶周梦洁许青霞刘发旺张英涛马明杨东辉北京大学医学部药学院天然药物及仿生药物国家重点实验室北京100191

Angucyclinones are aromatic polyketides produced by type Ⅱ polyketide synthases（PKS） and are mainly found in terrestrial actinomycetes. To discover more angucyclinones from marine actinomycetes, a genomic DNA-based PCR assay targeting type Ⅱ polyketide synthases was performed. Among the 167 marine actinomycetes strains screened, twelve strains were identified as the ＂positive＂ strains possessing type Ⅱ PKS-encoding genes based on the sequencing of PCR products. One of the 12 ＂positive＂ strains, Streptomyces sp. PKU-MA00218 was selected for the large-scale fermentation based on the HPLC and TLC analysis. Four angucyclinones, 6-deoxy-8-O-methylrabelomycin（1）, 8-O-methylrabelomycin（2）, 8-O-methyltetrangulol（3）, C-ring cleavage product of angucyclinone C（4）, were isolated and their structures were elucidated based on spectroscopic analyses. The isolation of angucyclinones 1–4 highlights the power of genome mining technologies based on biosynthetic knowledge in natural products discovery.

关键词： Angucyclinone Aromatic polyketides Genome mining Marine actinomycetes Type Ⅱ polyketide synthases

来源：评论

学校读者我要写书评

暂无评论

Selective alkylation and bioactivity of phosphorothioated nucleolin aptamer AS1411

引用

Journal of Chinese Pharmaceutical Sciences 2017年第1期26卷 23-30页

作者：张光普邓家荔杨先桃朱月洁关注张礼和杨振军北京大学医学部药学院天然药物及仿生药物国家重点实验室北京100191

An operationally simple and efficient method for the synthesis of a wide range of alkylated nucleotides under mild conditions was developed. This improved method furnishes alkylated nucleotides fi＇om both single nucleotides and oligonucleotides, and were prepared in high yields of 81% to 91%. Alkyl modified aptamer AS1411s were synthesized using this method and the biological activity screening results demonstrated that alkylation at the 1^st P-S site on yielded stronger target protein binding capacity, greater growth suppression effects against K562 and HL-60 cell lines, and improved serum stability, as compared with AS1411. This modified aptamer may be useful in tumor detection and treatment.

关键词： Phosphorothioated nucleotide Alkylation Aptamer AS 1411 Nucleolin

来源：评论

学校读者我要写书评

暂无评论

Phenanthrenes,anthraquinones,and phenolic constituents from Sinomenium acutum

引用

Journal of Chinese Pharmaceutical Sciences 2017年第6期26卷 440-446页

作者：吕海宁赵明波姜勇屠鹏飞北京大学医学部药学院天然药物及仿生药物国家重点实验室北京100191

A phytochemical investigation on the stems and rhizomes of Sinomenium acutum led to the isolation of 19 compounds, including three phenanthrenes, six anthraquinones, and ten phenolic derivatives. The structures of the isolated compounds were elucidated by analysis of the MS and NMR spectroscopic data and comparison with the literature. Compounds 1 and 2 are two new natural products, and their 1H and 13C NMR spectral data were fully assigned for the first time, with the help of2D NMR. All of the isolates were obtained from genus Sinomenium for the first time and the phenanthrene and anthraquinone are the structure type first reported from this genus plants.

关键词： Sinomenium acutum Phenanthrenes Anthraquinones Phenolic constituents

来源：评论

学校读者我要写书评

暂无评论

Quality standard study on Dictamni Cortex

引用

Journal of Chinese Pharmaceutical Sciences 2017年第4期26卷 298-303页

作者：王玲赵明波姜勇屠鹏飞郭晓宇北京大学医学部药学院天然药物及仿生药物国家重点实验室北京100191

As a traditional Chinese medicine （TCM）, Dictamni Cortex is widely used for the treatment of rheumatism and skin diseases. To promote the internationalization of Dictarnni Cortex, we established a recommended quality standard of Dictamni Cortex according to the European Pharmacopoeia （EP） in the present study. Foreign matter, loss on drying, total ash and ash insoluble in hydrochloric acid were determined according to the procedures of EP 8.0. The thin layer chromatography （TLC） identification method was established by using obacunone and fraxinellone as the reference substances. The content of dictarnnine, obacunone and fraxinellone was assayed by reversed phase high performance liquid chromatography （RP-HPLC）. Furthermore, we tested 21 batches of the prepared slices of Dictamni Cortex from different regions. The results indicated that the established quality standard was specific, accurate and internationalized, which could be used for the quality control of Dictamni Cortex.

关键词： Dictamni Cortex Quality standard European Pharmacopoeia HPLC

来源：评论

学校读者我要写书评

暂无评论

Hematological and pathological toxicity of anti-HER2/neu peptide mimetic modified paclitaxel bovine serum albumin nanoparticles

引用

Journal of Chinese Pharmaceutical Sciences 2013年第5期22卷 441-448页

作者：王占璋仰浈臻齐宪荣北京大学医学部药学院天然药物及仿生药物国家重点实验室北京100191

Nanoparticles have been widely applied in diagnosis and therapy due to the high loading of insoluble drug, increased target accumulation and interaction with biological tissues. Recently, severe side effects of nanoparticles have been reported, but the underlying mechanism remains largely unknown. In our study, we aim to understand the safety of paclitaxel （PTX） loaded bovine albumin nanoparticles （BNPs） and active targeted PTX loaded BNPs to normal vital organ or tissue in vivo. The anti-human epidermal growth factor receptor 2 （HER2/neu） peptide mimetic （AHNP） was covalent bound to surface of BNPs （AHNP-BNPs） to exert selected delivery to HER2＋ cells. In HER2＋ tumor xenographs, saline （control）, PTX traditional formula （medium of Cremophor EL-ethanol）, BNPs, and AHNP-BNPs were administrated to evaluate the toxicity. There is no severe neutropenia or anemia with treatment of BNPs and AHNP-BNPs compared with traditional PTX injection. We also evaluated their damage on normal organs, including liver, kidney, spleen, lung and heart to fully estimate the safety of AHNP-BNPs and BNPs delivery systems. We observed similar toxicity in liver and lung in mice treated with BNPs or PTX injection, but decreased liver damage in mice treated with AHNP-BNPs. Further studies are rcouired to confirm our conclusion.

关键词： Albumin nanoparticle Anti-HER2/neu peptide mimetic Target delivery Toxicity Paclitaxel

来源：评论

学校读者我要写书评

暂无评论

Fingerprint analysis of Ophiopogonis Radix by HPLC-UV-ELSD coupled with chemometrics methods

引用

Journal of Chinese Pharmaceutical Sciences 2013年第1期22卷 55-63页

作者：李宁车彦云张梁张加余周渊姜勇屠鹏飞北京大学医学部药学院天然药物及仿生药物国家重点实验室北京100191

A new, simple and reliable method using HPLC-UV-ELSD was developed to generate the fingerprint of Ophiopogonis Radix. Homoisoflavonoids and steroidal saponins were determined simultaneously in a single run. A total of 27 Ophiopogonis Radix samples were analyzed, and 18 reference substances were used for the identification of the common peaks. The fingerprint was further analyzed by chemometrics methods including similarity analysis （SA）, hierarchical clustering analysis （HCA） and principal component analysis （PCA）. The results indicated that the combination of chromatographic fingerprint and chemometrics analysis could be used for the geographical differentiation and quality evaluation of Ophiopognnis Radix.

关键词： Ophiopogonis Radix Ophiopogonjaponicus HPLC-UV-ELSD Fingerprint Chemometrics Quality evaluation

来源：评论

学校读者我要写书评

暂无评论

Discovery of thiazostatin siderphores from Streptomyces sp. HMU0027 with a genomic DNA-based PCR assay targeting nonribosomal peptide synthetase

引用

Journal of Chinese Pharmaceutical Sciences 2017年第10期26卷 737-746页

作者：刘发旺周梦洁金晶杨小燕张英涛马明杨东辉北京大学医学部药学院天然药物及仿生药物国家重点实验室北京100191

Nonribosomal peptides （NRPs） represent a large family of natural products with great diversities of chemical structures and biological activities. The peptide backbones of NRPs are synthesized by nonribosomal peptide synthetases （NRPSs） that minimally consist of one adenylation （A） domain, one peptidyl carrier protein （PCP） and one condensation （C） domain. In this study, we carded out a PCR screening and identified 21 ＂positive＂ strains from 100 actinomycete strains with the degenerate primers designed from the conserved sequences of A domains of NRPSs. One of the 21 ＂positive＂ strains, Streptomyces sp. HMU0027, was selected for large-scale fermentation （9 L） based on HPLC analysis, and subsequent isolation and structural elucidation afforded seven NRPS-synthesized thiazostatin siderophore analogues （1-7）. Compound 1 was a new compound containing an unusual linkage between a phenolate siderophore and a sugar moiety. These results laid the foundation for further biosynthetic research of these thiazostatin analogues and highlighted the power of genome mining technologies based on biosynthetic knowledge in NRP discovery.

关键词： Genome mining Nonribosomal peptide synthetase Siderophore Strain prioritization Thiazostation

来源：评论

学校读者我要写书评

暂无评论

Inhibitory effect of the combination of notoginseng total saponins and safflower total flavonoids on UDP-glucuronosyltransferases 1A1, 1A4, and 2B7 in human liver microsomes

引用

Journal of Chinese Pharmaceutical Sciences 2019年第4期28卷 229-237页

作者：李岩孟雨晴逯颖媛常坤高鹏姜勇屠鹏飞郭晓宇北京大学医学部药学院天然药物及仿生药物国家重点实验室北京100191

In the present study, the potential inhibition behaviors of notoginseng total saponins(NS), safflower total flavonoids(SF), and their combination(CNS) towards three major isoforms of UDP-glucuronosyltransferases(UGTs) in human liver microsomes(HLMs) were investigated to study the mechanism of the synergistic effect of ***, trifluoperazine and azidothymidine were selected as the probe drugs to elucidate the activities of UGT1A1, 1A4 and 2B7 by UPLC-MS/MS method, *** results showed that CNS, NS and SF significantly inhibited the activities of UGT1A1, 1A4 and 2B7(P<0.05) with the IC_(50) values less than 30 mg/***, the inhibitory effects of CNS towards UGT1A1, 1A4 and 2B7 were stronger than those of NS and SF(P<0.05).In conclusion, the combination of NS and SF could increase their inhibitory effects on UGT1A1, 1A4 and 2B7 activities in HLMs and might be conducive to reduce the phase II metabolism of the effective constituents in *** potential herb-drug interactions of CNS based on UGT enzymes provided a useful experimental basis for its further research and development.

关键词： UDP-glucuronosyltransferases Human liver microsomes Herb-drug interaction Notoginseng Radix et Rhizoma Carthami Flos

来源：评论

学校读者我要写书评

暂无评论

Pharmacophore identification and validation for human nAChR α7 agonists

引用

Journal of Chinese Pharmaceutical Sciences 2013年第5期22卷 393-402页

作者：于博金宏威张亮仁王超北京大学医学部药学院天然药物及仿生药物国家重点实验室北京100191

Human nAChR u7 is the potential target for schizophrenia cognitive disorders, and it is meaningful to develop selective human nAChR α7 agonists for the clinical treatment of the disease. Because the crystal structure ofα7 receptor has not been resolved, ligand-based drug design strategy was took in this work. A 3D QSAR pharmacophore model was built by HypoGen method, and its quality was evaluated by cost function. Furthermore, the pharmacophore model was validated with activity prediction of test set and was cross-validated based on Fisher＇s Randomization Method. By Enrichment Factor and AU-ROC analysis, the final pharmacophore, which is consisted of one HBA, two Hydrophobic and one PosIonizable, was selected and it fitted well with the docking result of α7 homology model and the ligand. The pharmacophore is expected for the following virtual screening and lead optimization of human nAChR α7 agonists, which is important for the development and discovery of novel antipsychotics.

关键词： Schizophrenia Human nAChR α7 Agonists Pharmacophore modeling Molecular docking

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：