间变型弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)是一种罕见的非特指型DLBCL,组织形态学常为窦性或者弥漫生长。该文报道1例,其左侧腋窝淋巴结具有大量多形性的中心母细胞样伴间变特征的细胞和HRS样细胞呈结节状或滤泡...
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间变型弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)是一种罕见的非特指型DLBCL,组织形态学常为窦性或者弥漫生长。该文报道1例,其左侧腋窝淋巴结具有大量多形性的中心母细胞样伴间变特征的细胞和HRS样细胞呈结节状或滤泡生发中心样生长,符合DLBCL,非特殊类型,间变型。结合相关文献探讨其临床病理学及分子遗传学特征,以提高临床和病理医师对该肿瘤的认识。
目的:探究丝裂原活化蛋白激酶10 (mitogen-activated protein kinase 10, MAPK10)和miR-335-5p在结直肠癌(colorectal cancer, CRC)中的表达及其两者之间的靶向结合关系。方法:本研究通过TCGA数据库分析CRC与正常结直肠组织中MAPK10和mi...
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目的:探究丝裂原活化蛋白激酶10 (mitogen-activated protein kinase 10, MAPK10)和miR-335-5p在结直肠癌(colorectal cancer, CRC)中的表达及其两者之间的靶向结合关系。方法:本研究通过TCGA数据库分析CRC与正常结直肠组织中MAPK10和miR-335-5p的转录水平表达差异,并在SW480和Caco2细胞系中提取RNA进行逆转录定量聚合酶链反应。此外,采用双荧光素酶实验验证miR-335-5p与MAPK10的靶向结合关系。结果:TCGA数据分析显示,CRC组织中MAPK10显著低表达(P Objective: To investigate the expression of mitogen-activated protein kinase 10 (MAPK10) and miR-335-5p in colorectal cancer (CRC) and their targeted binding relationship. Methods: In this study, the transcriptional level expression differences of MAPK10 and miR-335-5p between CRC and normal colorectal tissues were analyzed through the TCGA database. RNA was extracted from SW480 and Caco2 cell lines for reverse transcription quantitative polymerase chain reaction (RT-qPCR). Additionally, a dual-luciferase reporter assay was employed to verify the targeted binding relationship between miR-335-5p and MAPK10. Results: TCGA data analysis revealed that MAPK10 was significantly downregulated in CRC tissues (P < 0.05), while miR-335-5p was significantly upregulated (P < 0.001), and their expressions were negatively correlated (R = −0.246, P < 0.001). Cell line experiments confirmed the phenomena of low MAPK10 expression and high miR-335-5p expression. The dual-luciferase reporter assay demonstrated significant binding between miR-335-5p and the 3'untranslated region (3'UTR) of MAPK10 (P < 0.05). Conclusion: miR-335-5p inhibits the expression of MAPK10 by binding to its 3'UTR, suggesting its potential regulatory role in CRC.
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